用于血液培养中快速检测地中海斑疹热立克次体的离心管瓶技术
Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture.
作者信息
Marrero M, Raoult D
机构信息
Centre National de Reference des Rickettsioses, C.H.U. La Timone, Marseille, France.
出版信息
Am J Trop Med Hyg. 1989 Feb;40(2):197-9. doi: 10.4269/ajtmh.1989.40.197.
PMID:2645804
Abstract
The shell vial technique for isolation of cytomegalovirus was adapted to detect Rickettsia conorii in blood culture using human fibroblast monolayers. The inoculation was performed with low speed centrifugation and the rickettsiae demonstrated by immunofluorescence 24-120 hr after inoculation. R. conorii was identified in 11 of 13 patients with Mediterranean spotted fever in 48-72 hr.
摘要
用于分离巨细胞病毒的空斑小瓶技术经过改良，用于使用人成纤维细胞单层在血培养中检测康氏立克次体。接种通过低速离心进行，接种后24至120小时通过免疫荧光显示立克次体。在48至72小时内，13例地中海斑疹热患者中有11例检测出康氏立克次体。
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