Bi D Z
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Zhonghua Liu Xing Bing Xue Za Zhi. 1990 Aug;11(4):221-5.
One strain of spotted fever group (SFG) rickettsiae was isolated from a patient with febrile and headache who was missdiagnosed as common cold. The rickettsia was isolated by inoculation of yolk sacs of embryonated hen eggs with the patient's blood. The isolate was named as W-88 following the initial letter of the patient's name. It is the first time to report the isolation of SFG rickettsiae from human being who lived in city in China. In this study we compared the antigens of W-88 strain with seven prototype strains of SFG rickettsiae and six Chinese strains of SFG rickettsiae with one species-specific monoclonal antibody and one group reactive monoclonal antibody by indirect-immunofluorescence assay. The results demonstrated that W-88 strain and other Chinese strains JH-74, An-84, FT-84, MT-84, Se-85, To-85 of SFG rickettsiae were found identical to Rickettsia sibirica (strains 232 and 246) and differ from other prototypes of SFG rickettsiae.
从一名发热、头痛且被误诊为普通感冒的患者身上分离出一株斑点热群(SFG)立克次体。通过将患者血液接种到鸡胚卵黄囊中分离出该立克次体。按照患者姓名首字母将该分离株命名为W - 88。这是首次报道从中国城市居民中分离出SFG立克次体。在本研究中,我们用一种种特异性单克隆抗体和一种群反应性单克隆抗体,通过间接免疫荧光试验,将W - 88株的抗原与7株SFG立克次体原型株以及6株中国SFG立克次体株进行了比较。结果表明,W - 88株以及SFG立克次体的其他中国株JH - 74、An - 84、FT - 84、MT - 84、Se - 85、To - 85与西伯利亚立克次体(菌株232和246)相同,与SFG立克次体的其他原型株不同。