Zablocki Olivier, van Zyl Lonnie, Adriaenssens Evelien M, Rubagotti Enrico, Tuffin Marla, Cary Stephen C, Cowan Don
Centre for Microbial Ecology and Genomics; University of Pretoria ; Pretoria, South Africa ; Genome Research Institute; University of Pretoria ; Pretoria, South Africa.
Institute for Microbial Biotechnology and Metagenomics; University of the Western Cape ; Bellville, South Africa.
Bacteriophage. 2014 Dec 16;4(4):e980125. doi: 10.4161/21597081.2014.980125. eCollection 2014.
The metaviromes from 2 different Antarctic terrestrial soil niches have been analyzed. Both hypoliths (microbial assemblages beneath transluscent rocks) and surrounding open soils showed a high level diversity of tailed phages, viruses of algae and amoeba, and virophage sequences. Comparisons of other global metaviromes with the Antarctic libraries showed a niche-dependent clustering pattern, unrelated to the geographical origin of a given metavirome. Within the Antarctic open soil metavirome, a putative circularly permuted, ∼42kb dsDNA virus genome was annotated, showing features of a temperate phage possessing a variety of conserved protein domains with no significant taxonomic affiliations in current databases.
对来自2个不同南极陆地土壤生态位的宏病毒组进行了分析。隐生菌层(半透明岩石下的微生物群落)和周围的开阔土壤均显示出长尾噬菌体、藻类病毒、变形虫病毒和噬病毒体序列的高度多样性。将其他全球宏病毒组与南极文库进行比较,结果显示出一种生态位依赖性聚类模式,这与给定宏病毒组的地理起源无关。在南极开阔土壤宏病毒组中,注释了一个推定的环状排列的约42kb双链DNA病毒基因组,该基因组显示出温和噬菌体的特征,拥有多种保守蛋白结构域,在当前数据库中没有明显的分类学归属。