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南极病毒群落中特定生态位依赖的遗传多样性。

Niche-dependent genetic diversity in Antarctic metaviromes.

作者信息

Zablocki Olivier, van Zyl Lonnie, Adriaenssens Evelien M, Rubagotti Enrico, Tuffin Marla, Cary Stephen C, Cowan Don

机构信息

Centre for Microbial Ecology and Genomics; University of Pretoria ; Pretoria, South Africa ; Genome Research Institute; University of Pretoria ; Pretoria, South Africa.

Institute for Microbial Biotechnology and Metagenomics; University of the Western Cape ; Bellville, South Africa.

出版信息

Bacteriophage. 2014 Dec 16;4(4):e980125. doi: 10.4161/21597081.2014.980125. eCollection 2014.

Abstract

The metaviromes from 2 different Antarctic terrestrial soil niches have been analyzed. Both hypoliths (microbial assemblages beneath transluscent rocks) and surrounding open soils showed a high level diversity of tailed phages, viruses of algae and amoeba, and virophage sequences. Comparisons of other global metaviromes with the Antarctic libraries showed a niche-dependent clustering pattern, unrelated to the geographical origin of a given metavirome. Within the Antarctic open soil metavirome, a putative circularly permuted, ∼42kb dsDNA virus genome was annotated, showing features of a temperate phage possessing a variety of conserved protein domains with no significant taxonomic affiliations in current databases.

摘要

对来自2个不同南极陆地土壤生态位的宏病毒组进行了分析。隐生菌层(半透明岩石下的微生物群落)和周围的开阔土壤均显示出长尾噬菌体、藻类病毒、变形虫病毒和噬病毒体序列的高度多样性。将其他全球宏病毒组与南极文库进行比较,结果显示出一种生态位依赖性聚类模式,这与给定宏病毒组的地理起源无关。在南极开阔土壤宏病毒组中,注释了一个推定的环状排列的约42kb双链DNA病毒基因组,该基因组显示出温和噬菌体的特征,拥有多种保守蛋白结构域,在当前数据库中没有明显的分类学归属。

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