Grose Julianne H, Casjens Sherwood R
Microbiology and Molecular Biology Department, Brigham Young University, Provo, UT 84602, USA.
Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
Virology. 2014 Nov;468-470:421-443. doi: 10.1016/j.virol.2014.08.024.
Bacteriophages are the predominant biological entity on the planet. The recent explosion of sequence information has made estimates of their diversity possible. We describe the genomic comparison of 337 fully sequenced tailed phages isolated on 18 genera and 31 species of bacteria in the Enterobacteriaceae. These phages were largely unambiguously grouped into 56 diverse clusters (32 lytic and 24 temperate) that have syntenic similarity over >50% of the genomes within each cluster, but substantially less sequence similarity between clusters. Most clusters naturally break into sets of more closely related subclusters, 78% of which are correlated with their host genera. The largest groups of related phages are superclusters united by genome synteny to lambda (81 phages) and T7 (51 phages). This study forms a robust framework for understanding diversity and evolutionary relationships of existing tailed phages, for relating newly discovered phages and for determining host/phage relationships.
噬菌体是地球上占主导地位的生物实体。最近序列信息的激增使得对其多样性进行估计成为可能。我们描述了从肠杆菌科18个属和31种细菌中分离出的337个全序列有尾噬菌体的基因组比较。这些噬菌体在很大程度上被明确地分为56个不同的簇（32个裂解性和24个温和性），每个簇内超过50%的基因组具有共线性相似性，但不同簇之间的序列相似性要低得多。大多数簇自然地分解为关系更密切的子簇组，其中78%与它们的宿主属相关。最大的相关噬菌体组是通过与λ（81个噬菌体）和T7（51个噬菌体）的基因组共线性联合起来的超级簇。这项研究为理解现有有尾噬菌体的多样性和进化关系、关联新发现的噬菌体以及确定宿主/噬菌体关系形成了一个强大的框架。
