Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University , Beijing 100871, China.
Environ Sci Technol. 2015 Nov 17;49(22):13550-7. doi: 10.1021/acs.est.5b04002. Epub 2015 Oct 27.
The present study investigated long-term treatment performance and nitrogen transformation mechanisms in tidal flow constructed wetlands (TFCWs) under 4, 8, and 12 °C temperature regimes. High and stable ammonium (NH4(+)-N) removal efficiency (93-96%) was achieved in our TFCWs, whereas nitrate (NO3(-)-N) was accumulated at different levels under different temperatures. Quantitative response relationships showed anammox/amoA, (narG+napA)/amoA, and (narG+napA)/bacteria were the respective key functional gene groups determining 4, 8, and 12 °C NO3(-)-N reduction. Pathway analysis revealed the contribution of these functional gene groups along a depth gradient. In addition, denitrification process increased, while anammox process decreased consistent with a rise in temperature from 4 to 12 °C. Furthermore, cold temperatures exhibited different effects on anammox and denitrification and their long-term acclimatization capacities changed with temperature.
本研究探讨了在 4、8 和 12°C 温度条件下潮汐流人工湿地（TFCWs）的长期处理性能和氮转化机制。在我们的 TFCWs 中，实现了高且稳定的铵（NH4(+)-N）去除效率（93-96%），而硝酸盐（NO3(-)-N）在不同温度下积累到不同水平。定量响应关系表明，anammox/amoA、(narG+napA)/amoA 和 (narG+napA)/bacteria 分别是决定 4、8 和 12°C NO3(-)-N 还原的关键功能基因群。途径分析揭示了这些功能基因群沿深度梯度的贡献。此外，随着温度从 4°C 升高到 12°C，反硝化过程增加，而厌氧氨氧化过程减少。此外，低温对厌氧氨氧化和反硝化有不同的影响，它们的长期适应能力随温度变化而变化。
