Panayides A, Ioakeimidou A, Karamouzos V, Antonakos N, Koutelidakis I, Giannikopoulos G, Makaritsis K, Voloudakis N, Toutouzas K, Rovina N, Bristianou M, Damoraki G, Routsi C, Giamarellos-Bourboulis E J
Department of Nephrology, Nicosia General Hospital, Nicosia, Cyprus.
Intensive Care Unit, Korinthos General Hospital, Korinthos, Greece.
Eur J Clin Microbiol Infect Dis. 2015 Dec;34(12):2439-46. doi: 10.1007/s10096-015-2500-0. Epub 2015 Oct 10.
Single nucleotide polymorphisms (SNPs) of interleukin (IL)-6 are associated with the development of chronic renal disease (CRD). Their impact for sepsis in the field of CRD was investigated. One control cohort of 115 patients with CRD without infection and another case cohort of 198 patients with CRD and sepsis were enrolled. Genotyping at the -174 (rs1800795) and -572 positions of IL-6 (rs1800796) was done by restriction fragment length polymorphism. Circulating IL-6 was measured by an enzyme immunoassay. The GG genotype of rs1800796 was more frequent among cases (78.3%) than controls (62.6%). No difference in the genotype frequencies of rs1800795 between cases and controls were found. Odds ratio for sepsis was 2.07 (95%CI 1.24-3.44, p = 0.005) with the GG genotype of rs1800796, which was confirmed by logistic regression analysis taking into consideration the presence of chronic comorbidities. All-cause mortality until day 28 was similar between patients with the GG genotype and the GC/CC genotypes of rs1800796, but death caused from cardiovascular events not-related with infection was more frequent with the GG genotype (14.6% vs 2.4%, p = 0.031). Circulating IL-6 was greater among patients of the GC/CC genotypes of rs1800796 and multiple organ dysfunction (p = 0.013). The GG genotype of rs1800796 predisposes to sepsis in CRD and to 28-day mortality by sepsis-unrelated cardiovascular phenomena.
白细胞介素（IL）-6的单核苷酸多态性（SNP）与慢性肾病（CRD）的发生发展相关。本研究调查了其在CRD领域中对脓毒症的影响。纳入了一个由115例无感染的CRD患者组成的对照队列和另一个由198例患有CRD并伴有脓毒症的患者组成的病例队列。通过限制性片段长度多态性对IL-6基因-174（rs1800795）和-572位点（rs1800796）进行基因分型。采用酶免疫测定法检测循环中的IL-6。rs1800796的GG基因型在病例组（78.3%）中比对照组（62.6%）更常见。病例组和对照组之间rs1800795的基因型频率没有差异。rs1800796的GG基因型发生脓毒症的比值比为2.07（95%置信区间1.24 - 3.44，p = 0.005），在考虑慢性合并症存在的情况下，经逻辑回归分析得到证实。rs1800796的GG基因型患者与GC/CC基因型患者直至第28天的全因死亡率相似，但GG基因型患者因与感染无关的心血管事件导致的死亡更为常见（14.6%对2.4%，p = 0.031）。rs1800796的GC/CC基因型患者的循环IL-6水平更高且多器官功能障碍更为常见（p = 0.013）。rs1800796的GG基因型易导致CRD患者发生脓毒症，并因与脓毒症无关的心血管现象导致28天死亡率升高。
