长链非编码RNA SPRY4-IT1的敲低抑制胶质瘤细胞增殖、转移及上皮-间质转化。
Knockdown of long noncoding RNA SPRY4-IT1 suppresses glioma cell proliferation, metastasis and epithelial-mesenchymal transition.
作者信息
Liu Hongjiang, Lv Zhongqiang, Guo Erkun
机构信息
Department of Neurosurgery, The Second Hospital of Hebei Medical University Shijiazhuang 050000, Hebei, China.
出版信息
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9140-6. eCollection 2015.
Abstract
Long noncoding RNAs (lncRNAs), a class of ribonucleic molecules, participate in various cellular processes. They are highly expressed in several types of cancer and their expression was related to pathophysiological characteristics of tumor growth, therefore, they can be considered as a promising diagnostic tool and a convenient prognostic biomarker. SPRY4-IT1, belonging to a group of intron-retained lncRNAs, was reported to affect tumor development of many types of cancer. However, the expression and the role of SPRY4-IT1 in glioma are still unclear. Therefore, in this study, we examined for the first time the expression and role of SPRY4-IT1 in glioma cells. The results of our study showed that SPRY4-IT1 was up-regulated in human glioma tissues and cell lines. Knockdown of SPRY4-IT1 could inhibit glioma cell growth and migration. Moreover, knockdown of SPRY4-IT1 could inhibit epithelial-mesenchymal transition (EMT) phenotype in glioma cells. Based on these findings, SPRY4-IT1 may be used as a new target for diagnosis and treatment of glioma.
摘要
长链非编码RNA（lncRNAs）是一类核糖核酸分子，参与多种细胞过程。它们在多种癌症类型中高表达，且其表达与肿瘤生长的病理生理特征相关，因此，它们可被视为一种有前景的诊断工具和便捷的预后生物标志物。SPRY4-IT1属于一组内含子保留lncRNAs，据报道其会影响多种癌症的肿瘤发展。然而，SPRY4-IT1在胶质瘤中的表达及作用仍不清楚。因此，在本研究中，我们首次检测了SPRY4-IT1在胶质瘤细胞中的表达及作用。我们的研究结果表明，SPRY4-IT1在人胶质瘤组织和细胞系中上调。敲低SPRY4-IT1可抑制胶质瘤细胞的生长和迁移。此外，敲低SPRY4-IT1可抑制胶质瘤细胞的上皮-间质转化（EMT）表型。基于这些发现，SPRY4-IT1可能用作胶质瘤诊断和治疗的新靶点。
相似文献
1
Knockdown of long noncoding RNA SPRY4-IT1 suppresses glioma cell proliferation, metastasis and epithelial-mesenchymal transition.长链非编码RNA SPRY4-IT1的敲低抑制胶质瘤细胞增殖、转移及上皮-间质转化。
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9140-6. eCollection 2015.
2
Decreased long non-coding RNA SPRY4-IT1 contributes to ovarian cancer cell metastasis partly via affecting epithelial-mesenchymal transition.长链非编码RNA SPRY4-IT1表达降低部分通过影响上皮-间质转化促进卵巢癌细胞转移。
Tumour Biol. 2017 Jul;39(7):1010428317709129. doi: 10.1177/1010428317709129.
3
Decreased long noncoding RNA SPRY4-IT1 contributing to gastric cancer cell metastasis partly via affecting epithelial-mesenchymal transition.长链非编码RNA SPRY4-IT1表达降低部分通过影响上皮-间质转化促进胃癌细胞转移。
J Transl Med. 2015 Aug 4;13:250. doi: 10.1186/s12967-015-0595-9.
4
Upregulation of long noncoding RNA SPRY4-IT1 promotes metastasis of esophageal squamous cell carcinoma via induction of epithelial-mesenchymal transition.长链非编码 RNA SPRY4-IT1 的上调通过诱导上皮-间充质转化促进食管鳞状细胞癌的转移。
Cell Biol Toxicol. 2016 Oct;32(5):391-401. doi: 10.1007/s10565-016-9341-1. Epub 2016 Jun 2.
5
Overexpression of the long non-coding RNA SPRY4-IT1 promotes tumor cell proliferation and invasion by activating EZH2 in hepatocellular carcinoma.长链非编码RNA SPRY4-IT1的过表达通过激活肝细胞癌中的EZH2促进肿瘤细胞增殖和侵袭。
Biomed Pharmacother. 2017 Jan;85:348-354. doi: 10.1016/j.biopha.2016.11.035. Epub 2016 Nov 28.
6
Long non-coding RNA SPRY4-IT1 promotes proliferation and invasion by acting as a ceRNA of miR-101-3p in colorectal cancer cells.长链非编码RNA SPRY4-IT1通过作为miR-101-3p的竞争性内源RNA促进结肠癌细胞的增殖和侵袭。
Tumour Biol. 2017 Jul;39(7):1010428317716250. doi: 10.1177/1010428317716250.
7
Long non-coding RNA SPRY4-IT1 promotes gallbladder carcinoma progression.长链非编码RNA SPRY4-IT1促进胆囊癌进展。
Oncotarget. 2017 Jan 10;8(2):3104-3110. doi: 10.18632/oncotarget.13621.
8
Long noncoding RNA SPRY4-IT1 is a prognostic factor for poor overall survival and has an oncogenic role in glioma.长链非编码RNA SPRY4-IT1是总生存期较差的一个预后因素，并且在胶质瘤中具有致癌作用。
Eur Rev Med Pharmacol Sci. 2016 Jul;20(14):3035-9.
9
SPRY4-IT1: A novel oncogenic long non-coding RNA in human cancers.SPRY4-IT1：一种人类癌症中的新型致癌长链非编码RNA。
Tumour Biol. 2017 Jun;39(6):1010428317711406. doi: 10.1177/1010428317711406.
10
Increased expression of SPRY4-IT1 predicts poor prognosis and promotes tumor growth and metastasis in bladder cancer.SPRY4-IT1表达增加预示着膀胱癌预后不良，并促进肿瘤生长和转移。
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1954-60. eCollection 2015.
引用本文的文献
1
Diagnostics and Therapy for Malignant Tumors.恶性肿瘤的诊断与治疗
Biomedicines. 2024 Nov 21;12(12):2659. doi: 10.3390/biomedicines12122659.
2
Regulation of EMT Markers, Extracellular Matrix, and Associated Signalling Pathways by Long Non-Coding RNAs in Glioblastoma Mesenchymal Transition: A Scoping Review.长链非编码RNA对胶质母细胞瘤间充质转化中EMT标志物、细胞外基质及相关信号通路的调控：一项综述
Biology (Basel). 2023 Jun 4;12(6):818. doi: 10.3390/biology12060818.
3
The Mechanisms of Current Platinum Anticancer Drug Resistance in the Glioma.脑胶质瘤中当前铂类抗癌药物耐药的机制。
Curr Pharm Des. 2022;28(23):1863-1869. doi: 10.2174/1381612828666220607105746.
4
Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.耐药性和敏感性长链非编码 RNA，SNHG1 和 UBL7-AS1 促进脑胶质母细胞瘤细胞增殖。
Oxid Med Cell Longev. 2022 Mar 11;2022:2623599. doi: 10.1155/2022/2623599. eCollection 2022.
5
A Review on the Role of SPRY4-IT1 in the Carcinogenesis.SPRY4-IT1在肿瘤发生中的作用综述
Front Oncol. 2022 Jan 13;11:779483. doi: 10.3389/fonc.2021.779483. eCollection 2021.
6
Non-coding RNAs and glioblastoma: Insight into their roles in metastasis.非编码RNA与胶质母细胞瘤：深入了解它们在转移中的作用
Mol Ther Oncolytics. 2021 Dec 22;24:262-287. doi: 10.1016/j.omto.2021.12.015. eCollection 2022 Mar 17.
7
Non-coding RNAs in melanoma: Biological functions and potential clinical applications.黑色素瘤中的非编码RNA：生物学功能及潜在临床应用
Mol Ther Oncolytics. 2021 Jun 4;22:219-231. doi: 10.1016/j.omto.2021.05.012. eCollection 2021 Sep 24.
8
Long Noncoding RNA NONHSAT079852.2 Contributes to GBM Recurrence by Functioning as a ceRNA for has-mir-10401-3p to Facilitate HSPA1A Upregulation.长链非编码RNA NONHSAT079852.2通过作为has-mir-10401-3p的竞争性内源RNA发挥作用促进HSPA1A上调，从而导致胶质母细胞瘤复发。
Front Oncol. 2021 Jul 8;11:636632. doi: 10.3389/fonc.2021.636632. eCollection 2021.
9
Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy.Meg3通过自噬诱导胶质瘤细胞的上皮-间质转化和侵袭。
Onco Targets Ther. 2020 Jan 31;13:989-1000. doi: 10.2147/OTT.S239648. eCollection 2020.
10
Eupatilin inhibits glioma proliferation, migration, and invasion by arresting cell cycle at G1/S phase and disrupting the cytoskeletal structure.紫茎泽兰苷通过将细胞周期阻滞在G1/S期并破坏细胞骨架结构来抑制胶质瘤的增殖、迁移和侵袭。
Cancer Manag Res. 2019 May 24;11:4781-4796. doi: 10.2147/CMAR.S207257. eCollection 2019.
本文引用的文献
1
The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression.长链非编码RNA SPRY4-IT1通过上调ZNF703表达来增加人乳腺癌细胞的增殖。
Mol Cancer. 2015 Feb 22;14:51. doi: 10.1186/s12943-015-0318-0.
2
The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells.人黑色素瘤细胞中长链非编码RNA SPRY4-IT1的功能表征
Oncotarget. 2014 Oct 15;5(19):8959-69. doi: 10.18632/oncotarget.1863.
3
High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma.长链非编码RNA SPRY4-IT1的高表达预示着透明细胞肾细胞癌的预后不良。
Int J Clin Exp Pathol. 2014 Aug 15;7(9):5801-9. eCollection 2014.
4
Long noncoding RNA MALAT1 promotes brain metastasis by inducing epithelial-mesenchymal transition in lung cancer.长链非编码RNA MALAT1通过诱导肺癌上皮-间质转化促进脑转移。
J Neurooncol. 2015 Jan;121(1):101-8. doi: 10.1007/s11060-014-1613-0. Epub 2014 Sep 14.
5
Long noncoding RNA HOTAIR involvement in cancer.长链非编码RNA HOTAIR与癌症的关系
Tumour Biol. 2014 Oct;35(10):9531-8. doi: 10.1007/s13277-014-2523-7. Epub 2014 Aug 29.
6
BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition.BRAF激活的长链非编码RNA通过诱导上皮-间质转化促进结直肠癌迁移。
Oncol Lett. 2014 Aug;8(2):869-875. doi: 10.3892/ol.2014.2154. Epub 2014 May 19.
7
Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis.长链非编码RNA SPRY4-IT1在食管鳞状细胞癌中上调并与不良预后相关。
Tumour Biol. 2014 Aug;35(8):7743-54. doi: 10.1007/s13277-014-2013-y. Epub 2014 May 9.
8
A long non-coding RNA signature to improve prognosis prediction of colorectal cancer.一种用于改善结直肠癌预后预测的长链非编码RNA特征
Oncotarget. 2014 Apr 30;5(8):2230-42. doi: 10.18632/oncotarget.1895.
9
Glioma and glioblastoma - how much do we (not) know?神经胶质瘤和胶质母细胞瘤——我们（不）了解多少？
Mol Clin Oncol. 2013 Nov;1(6):935-941. doi: 10.3892/mco.2013.172. Epub 2013 Aug 26.
10
The controversial role of microglia in malignant gliomas.小胶质细胞在恶性胶质瘤中的争议性作用。
Clin Dev Immunol. 2013;2013:285246. doi: 10.1155/2013/285246. Epub 2013 Jul 24.
Zotero 插件