Liu Hongjiang, Lv Zhongqiang, Guo Erkun
Department of Neurosurgery, The Second Hospital of Hebei Medical University Shijiazhuang 050000, Hebei, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9140-6. eCollection 2015.
Long noncoding RNAs (lncRNAs), a class of ribonucleic molecules, participate in various cellular processes. They are highly expressed in several types of cancer and their expression was related to pathophysiological characteristics of tumor growth, therefore, they can be considered as a promising diagnostic tool and a convenient prognostic biomarker. SPRY4-IT1, belonging to a group of intron-retained lncRNAs, was reported to affect tumor development of many types of cancer. However, the expression and the role of SPRY4-IT1 in glioma are still unclear. Therefore, in this study, we examined for the first time the expression and role of SPRY4-IT1 in glioma cells. The results of our study showed that SPRY4-IT1 was up-regulated in human glioma tissues and cell lines. Knockdown of SPRY4-IT1 could inhibit glioma cell growth and migration. Moreover, knockdown of SPRY4-IT1 could inhibit epithelial-mesenchymal transition (EMT) phenotype in glioma cells. Based on these findings, SPRY4-IT1 may be used as a new target for diagnosis and treatment of glioma.
长链非编码RNA（lncRNAs）是一类核糖核酸分子，参与多种细胞过程。它们在多种癌症类型中高表达，且其表达与肿瘤生长的病理生理特征相关，因此，它们可被视为一种有前景的诊断工具和便捷的预后生物标志物。SPRY4-IT1属于一组内含子保留lncRNAs，据报道其会影响多种癌症的肿瘤发展。然而，SPRY4-IT1在胶质瘤中的表达及作用仍不清楚。因此，在本研究中，我们首次检测了SPRY4-IT1在胶质瘤细胞中的表达及作用。我们的研究结果表明，SPRY4-IT1在人胶质瘤组织和细胞系中上调。敲低SPRY4-IT1可抑制胶质瘤细胞的生长和迁移。此外，敲低SPRY4-IT1可抑制胶质瘤细胞的上皮-间质转化（EMT）表型。基于这些发现，SPRY4-IT1可能用作胶质瘤诊断和治疗的新靶点。
