Zhu Jianyou, Wang Rugang, Cao Hongyun, Zhang Haipeng, Xu Shaoyan, Wang Aiyun, Liu Bin, Wang Yongtao, Wang Ruicai
Department of Pathology, People's Hospital of Linzi District, Affiliated to Binzhou Medical College Zibo, Shandong Province, China.
Department of Clinical Laboratory, People's Hospital of Linzi District, Affiliated to Binzhou Medical College Zibo, Shandong Province, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9479-86. eCollection 2015.
Recent data indicate that the tight junction proteins are abnormally regulated in several human cancers and the expression of these proteins is involved in the etiology and progression of cancer. To explore the expression distinction of the tight junction proteins claudin-5, -7, -8 and -9 in the adjacent non-neoplastic tissues and cervical carcinoma tissues, 72 cervical carcinoma tissues and the samples of non-neoplastic tissues adjacent to the tumors were examined for expression of claudin-5, -7, -8 and -9 by streptavidin-perosidase immunohistochemical staining method. The positive expression rates of claudin-5 in cervical carcinoma tissues and adjacent non-neoplastic tissues were 31.9% (23/72) and 51.4% (37/72) respectively (P < 0.05). The positive expression rates of claudin-7 in cervical carcinoma tissues and adjacent non-neoplastic tissues were 47.2% and 50.0% respectively (P = 1.000). The positive expression rates of claudin-8 in cervical carcinoma tissues and adjacent non-neoplastic tissues were 54.2 % and 27.8% respectively (P < 0.01). The positive expression rates of claudin-9 in cervical carcinoma tissues and adjacent non-neoplastic tissues were 38.9% and 56.9% respectively (P < 0.05). Thus in our study, the expression of claudin-5 and claudin-9 was down-regulated while the expression of claudin-8 was up-regulated in cervical carcinoma tissues compared with adjacent non-neoplastic tissues. The expression of claudin-7 has no obviously difference between cervical carcinoma tissues and adjacent non-neoplastic tissues. In addition, correlations between claudin-5, -8 and -9 expression with lymphatic metastasis were observed. Our study reveals that the expression of claudin-5, -8 and -9 altered between in cervical carcinoma tissues and adjacent non-neoplastic tissues.
近期数据表明，紧密连接蛋白在多种人类癌症中存在异常调控，且这些蛋白的表达与癌症的病因及进展有关。为探究紧密连接蛋白claudin - 5、- 7、- 8和- 9在相邻非肿瘤组织和宫颈癌组织中的表达差异，采用链霉亲和素-过氧化物酶免疫组织化学染色法检测了72例宫颈癌组织及肿瘤相邻的非肿瘤组织样本中claudin - 5、- 7、- 8和- 9的表达。claudin - 5在宫颈癌组织和相邻非肿瘤组织中的阳性表达率分别为31.9%（23/72）和51.4%（37/72）（P < 0.05）。claudin - 7在宫颈癌组织和相邻非肿瘤组织中的阳性表达率分别为47.2%和50.0%（P = 1.000）。claudin - 8在宫颈癌组织和相邻非肿瘤组织中的阳性表达率分别为54.2%和27.8%（P < 0.01）。claudin - 9在宫颈癌组织和相邻非肿瘤组织中的阳性表达率分别为38.9%和56.9%（P < 0.05）。因此，在我们的研究中，与相邻非肿瘤组织相比，宫颈癌组织中claudin - 5和claudin - 9的表达下调，而claudin - 8的表达上调。claudin - 7在宫颈癌组织和相邻非肿瘤组织中的表达无明显差异。此外，还观察到claudin - 5、- 8和- 9的表达与淋巴转移之间的相关性。我们的研究表明，claudin - 5、- 8和- 9在宫颈癌组织和相邻非肿瘤组织中的表达存在改变。
