Arab Mohammad Reza, Mirzaei Ramazan, Aval Fereydoon Sargolzaei
Cell and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Health Promotion Research Center, Faculty of Health, Zahedan University of Medical Sciences, Zahedan, Iran.
Cell J. 2015 Fall;17(3):422-8. doi: 10.22074/cellj.2015.3. Epub 2015 Oct 7.
The aim of the present study was to evaluate the protective effects of gadoli- num on pneumotoxic effects of styrene in rats as an experimental model.
In this experimental study a total number of 40 adult male Sprague Dawley rats that weighed 200 ± 13 g were randomly divided into five groups: i. styrene (St, N=10), ii. styrene+gadolinium chloride (GdCl3, N=10), iii. control (N=10), iv. GdCl3 (N=5) and v. normal saline (Nor.Sal, as a solvent of GdCl3, N=5). Normal saline, as a sham control group, was otherwise treated identically. Rats from the experimental groups were exposed to St in an exposure chamber for 6 days/week, 4 hours/day for up to 3 weeks. At the end of the experi- ment, rats from all groups were killed by deep anesthesia. Their lungs were removed, then fixed in formalin and weighed. Tissue samples were processed routinely and sections stained by the hematoxylin and eosin (H&E) and periodic acid Schiff (PAS) methods. We measured the thicknesses of the respiratory epithelia and interalveolar septa. Obtained data were ana- lyzed by ANOVA, the Tukey test and the paired t test.
Shedding of apical cytoplasm in the bronchiole was a prominent feature of the St group. PAS staining revealed histochemical changes in goblet cells in the epithelium of the St group. While there were no significant changes in lung weights and respiratory epithelial thicknesses between all studied groups, statistical analysis showed a significant alteration in the thickness of interalveolar septa in the St and St+GdCl3 group compared to the control groups (P<0.001).
Styrene induced structural and histochemical changes in bronchiole, interalveolar septa and alveolar organization in the rats' lungs. Gadolinium appeared to partially reduce the toxic effects of styrene on the lungs.
本研究旨在以大鼠为实验模型，评估钆对苯乙烯肺毒性作用的保护效果。
在本实验研究中，将40只体重为200±13克的成年雄性斯普拉格-道利大鼠随机分为五组：i. 苯乙烯组（St，N = 10）；ii. 苯乙烯+氯化钆组（GdCl3，N = 10）；iii. 对照组（N = 10）；iv. 氯化钆组（N = 5）；v. 生理盐水组（Nor.Sal，作为GdCl3的溶剂，N = 5）。生理盐水组作为假对照组，其他处理方式相同。实验组大鼠在暴露舱中每周暴露于苯乙烯6天，每天4小时，持续3周。实验结束时，所有组的大鼠通过深度麻醉处死。取出它们的肺，然后用福尔马林固定并称重。组织样本常规处理，切片用苏木精和伊红（H&E）以及过碘酸希夫（PAS）法染色。我们测量了呼吸上皮和肺泡间隔的厚度。所得数据通过方差分析、Tukey检验和配对t检验进行分析。
细支气管顶端细胞质脱落是苯乙烯组的一个显著特征。PAS染色显示苯乙烯组上皮中杯状细胞的组织化学变化。虽然所有研究组之间肺重量和呼吸上皮厚度没有显著变化，但统计分析表明，与对照组相比，苯乙烯组和苯乙烯+氯化钆组的肺泡间隔厚度有显著改变（P < 0.001）。
苯乙烯诱导了大鼠肺中细支气管、肺泡间隔和肺泡组织的结构和组织化学变化。钆似乎部分降低了苯乙烯对肺的毒性作用。
