Sammra Osama, Balbutskaya Anna, Hijazin Muaz, Nagib Samy, Alber Jörg, Lämmler Christoph, Abdulmawjood Amir, Prenger-Berninghoff Ellen, Timke Markus, Kostrzewa Markus, Siebert Ursula
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.
Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
J Vet Med. 2014;2014:923592. doi: 10.1155/2014/923592. Epub 2014 Feb 6.
Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.
拟海豹隐秘杆菌是一种新描述的物种,其模式菌株拟海豹隐秘杆菌2698(T) 是从一只斑海豹的阴道拭子中分离得到的,另外还有四株拟海豹隐秘杆菌菌株也从四只斑海豹中分离得到,通过表型特征、基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS) 以及对基因组靶点16S rDNA、16S-23S rDNA基因间隔区以及rpoB和gap基因进行测序,能够可靠地鉴定出这些菌株。在本研究中所调查的拟海豹隐秘杆菌菌株是与其他几种细菌一起分离得到的,这表明拟海豹隐秘杆菌的致病重要性仍不清楚。然而,通过MALDI-TOF MS检测肽谱以及本文所呈现的表型和基因型方法可能有助于未来对拟海豹隐秘杆菌的鉴定。