Saracut Claudiu, Molnar Calin, Russu Cristian, Todoran Nicoleta, Vlase Laurian, Turdean Sabin, Voidazan Septimiu, Copotoiu Constantin
Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
Department of Pharmaceutical Technology, University of Medicine and Pharmacy Tg-Mures, RO.
Acta Cir Bras. 2015 Sep;30(9):624-31. doi: 10.1590/S0102-865020150090000007.
To assess whether deoxycholic acid (DOC) and lithocholic acid (LCA) administered in a period of six months in a concentration of 0.25% may have a carcinogenic role in mice colon.
The study used C57BL6 female mice divided into four groups. The control group received a balanced diet and the others received diets supplemented with 0.25% DOC, 0.25% LCA and 0.125% DOC+0.125% LCA, respectively. After euthanasia, the lesions found in the resected gastrointestinal tracts were stained with hematoxylin-eosin and examined microscopically.
No gastrointestinal tract changes were observed in the control group, while hyperplastic Peyer's patches in the small intestine, flat adenomas with mild dysplasia and chronic colitis at the level of the colon were found in all three test groups. The colonic lesions prevailed in the proximal colon. The highest number of flat adenoma lesions (8), hyperplasia of Peyer's patches (25) and chronic colitis (2) were found in mice fed with diet and LCA.
Precancerous or cancerous pathological lesions could not be identified. Instead, adenomatous colonic injuries occurred in a shorter period of time (six months), compared to the reported data.
评估在六个月的时间内以0.25%的浓度给予脱氧胆酸（DOC）和石胆酸（LCA）是否可能对小鼠结肠产生致癌作用。
该研究使用了C57BL6雌性小鼠，分为四组。对照组给予均衡饮食，其他组分别给予补充有0.25% DOC、0.25% LCA和0.125% DOC + 0.125% LCA的饮食。安乐死后，对切除的胃肠道中发现的病变进行苏木精-伊红染色并进行显微镜检查。
对照组未观察到胃肠道变化，而在所有三个试验组中均发现小肠中派伊尔结增生、轻度发育异常的扁平腺瘤以及结肠水平的慢性结肠炎。结肠病变在近端结肠最为常见。在喂食含LCA饮食的小鼠中发现扁平腺瘤病变数量最多（8个）、派伊尔结增生（25个）和慢性结肠炎（2个）。
未发现癌前或癌性病理病变。相反，与报告数据相比，在较短时间（六个月）内出现了结肠腺瘤性损伤。
