文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

γ-介导的肠道微生物群抗生素后重建对小鼠结肠炎相关结直肠癌发生发展的负面影响。

The negative effect of -mediated post-antibiotic reconstitution of the gut microbiota on the development of colitis-associated colorectal cancer in mice.

作者信息

Wang Kaicen, Wu Wenrui, Wang Qing, Yang Liya, Bian Xiaoyuan, Jiang Xianwan, Lv Longxian, Yan Ren, Xia Jiafeng, Han Shengyi, Li Lanjuan

机构信息

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China.

出版信息

Front Microbiol. 2022 Oct 14;13:932047. doi: 10.3389/fmicb.2022.932047. eCollection 2022.

DOI:10.3389/fmicb.2022.932047
PMID:36312913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9614165/
Abstract

The bidirectional relationship between colorectal cancer (CRC) and the gut microbiome has been well-documented. Here, we investigated the impact of -mediated post-antibiotic gut microbial reconstitution on the development of colitis-associated CRC (CAC). The results showed that post-antibiotic replenishment of worsened the tumorigenesis of CAC as indicated by increased number of large (>2 mm in diameter) tumors and both average and total tumor diameters. Measures of intestinal barrier function showed that post-antibiotic gavage damaged the intestinal barrier as reflected by lower transcriptional levels of , and . Impaired gut barrier was followed by lipopolysaccharides (LPS) translocation as indicated by higher level of serum LPS-binding protein (LBP). The increased colonic mRNA levels of , and and serum levels of IL-1β, IL-6, and TNF-α indicated that post-antibiotic replenishment resulted in overactivated inflammatory environment in CAC. The analysis of the evolution of the microbial community during the progression of CAC showed that post-antibiotic supplementation of led to a distinct microbial configuration when compared with other treatments characterized by enriched Firmicutes, Lachnospiraceae, and Ruminococcaceae, and depleted Bacteroidetes, which was accompanied by higher Firmicutes/Bacteroidetes (F/B) ratio. Furthermore, post-antibiotic administration changed the bile acid (BA) metabolic profile as indicated by decreased concentrations of secondary BA (SBA), ω-murocholic acid (ωMCA), and murocholic acid (muroCA). In addition, the supplementation after antibiotic pretreatment also impacted the metabolism of short-chain fatty acids (SCFAs) as evidenced by increased concentrations of acetic acid, propionic acid, butyric acid, and valeric acid. Our study surprisingly observed that -mediated post-antibiotic reconstitution of the gut microbiota aggravated the CAC in mice. It might exert its effect by damaging the gut barrier, exacerbating inflammatory responses, disrupting the post-antibiotic recovery of the microbial community, and further influencing the metabolism of BA and SCFAs. These findings indicated that maintaining the homeostasis of intestinal microorganisms is more crucial to health than replenishing a single beneficial microbe, and probiotics should be used with caution after antibiotic treatment.

摘要

结直肠癌（CRC）与肠道微生物群之间的双向关系已有充分记录。在此，我们研究了介导的抗生素后肠道微生物重建对结肠炎相关结直肠癌（CAC）发生发展的影响。结果表明，抗生素后补充会恶化CAC的肿瘤发生，表现为直径大于2毫米的大肿瘤数量增加，以及平均肿瘤直径和总肿瘤直径均增加。肠道屏障功能的检测表明，抗生素后灌胃会损害肠道屏障，表现为、和的转录水平降低。肠道屏障受损后，脂多糖（LPS）发生易位，表现为血清LPS结合蛋白（LBP）水平升高。结肠中、和的mRNA水平升高以及血清中IL-1β、IL-6和TNF-α水平升高表明，抗生素后补充会导致CAC中炎症环境过度激活。对CAC进展过程中微生物群落演变的分析表明，与其他以厚壁菌门、毛螺菌科和瘤胃球菌科富集以及拟杆菌门减少为特征的治疗相比，抗生素后补充会导致独特的微生物结构，同时伴有更高的厚壁菌门/拟杆菌门（F/B）比率。此外，抗生素后给药改变了胆汁酸（BA）代谢谱，表现为次级BA（SBA）、ω-鼠胆酸（ωMCA）和鼠胆酸（muroCA）浓度降低。此外，抗生素预处理后补充也影响了短链脂肪酸（SCFA）的代谢，表现为乙酸、丙酸、丁酸和戊酸浓度增加。我们的研究令人惊讶地发现，介导的抗生素后肠道微生物群重建会加重小鼠的CAC。它可能通过破坏肠道屏障、加剧炎症反应、扰乱抗生素后微生物群落的恢复以及进一步影响BA和SCFA的代谢来发挥作用。这些发现表明，维持肠道微生物的稳态对健康比补充单一有益微生物更为关键，抗生素治疗后应谨慎使用益生菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/1920f0039fb4/fmicb-13-932047-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/834ecaaf1a69/fmicb-13-932047-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/26da742711cd/fmicb-13-932047-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/3c002ea8e372/fmicb-13-932047-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/ac931280e85e/fmicb-13-932047-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/608755cb00fe/fmicb-13-932047-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/ccf84785065d/fmicb-13-932047-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/830c10b301ab/fmicb-13-932047-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/8d0da36bac97/fmicb-13-932047-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/1920f0039fb4/fmicb-13-932047-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/834ecaaf1a69/fmicb-13-932047-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/26da742711cd/fmicb-13-932047-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/3c002ea8e372/fmicb-13-932047-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/ac931280e85e/fmicb-13-932047-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/608755cb00fe/fmicb-13-932047-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/ccf84785065d/fmicb-13-932047-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/830c10b301ab/fmicb-13-932047-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/8d0da36bac97/fmicb-13-932047-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d844/9614165/1920f0039fb4/fmicb-13-932047-g009.jpg

相似文献

[1]
The negative effect of -mediated post-antibiotic reconstitution of the gut microbiota on the development of colitis-associated colorectal cancer in mice.

Front Microbiol. 2022-10-14

[2]
Protective Effect of against Immune-Mediated Liver Injury in a Mouse Model.

Front Microbiol. 2017-9-26

[3]
Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.

Phytomedicine. 2022-7-20

[4]
and synergistically protect from colitis by promoting ILC3 in the gut.

mBio. 2024-4-10

[5]
Akkermansia muciniphila Ameliorates Acetaminophen-Induced Liver Injury by Regulating Gut Microbial Composition and Metabolism.

Microbiol Spectr. 2022-2-23

[6]
Protects Against Psychological Disorder-Induced Gut Microbiota-Mediated Colonic Mucosal Barrier Damage and Aggravation of Colitis.

Front Cell Infect Microbiol. 2021

[7]
Administration of Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.

Front Microbiol. 2019-10-1

[8]
A purified membrane protein from or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8 T cells in mice.

Gut. 2020-11

[9]
Impact of grape polyphenols on and the gut barrier.

AIMS Microbiol. 2022-12-22

[10]
TLR4 regulates RORγt regulatory T-cell responses and susceptibility to colon inflammation through interaction with Akkermansia muciniphila.

Microbiome. 2022-6-27

引用本文的文献

[1]
Selenium-enriched Lactobacillus coryniformis H8 alleviates LPS-induced jejunal injury in chicks by modulating antioxidant activity, inflammation, selenoprotein genes, and gut microbiota.

Poult Sci. 2025-8-13

[2]
An investigation for phylogenetic characterization of human pancreatic cancer microbiome by 16S rDNA sequencing and bioinformatics techniques.

J Solid Tumors. 2024

[3]
Effect of Dealcoholized Muscadine Wine on the Development of Spontaneous Colitis and Gut Microbiome in IL-10 Mice.

Nutrients. 2025-7-16

[4]
The Role of Akkermansia muciniphila in Disease Regulation.

Probiotics Antimicrob Proteins. 2025-7-9

[5]
The interaction network between intestinal flora and cell death in microecosystem of pan-cancers.

Int J Med Sci. 2025-5-16

[6]
Preliminary investigation of gut microbiota and associated metabolic pathways in the pathogenesis of primary central nervous system lymphoma.

Front Oncol. 2025-4-2

[7]
The gut microbiota: an emerging modulator of drug resistance in hepatocellular carcinoma.

Gut Microbes. 2025-12

[8]
Gut dysbiosis was inevitable, but tolerance was not: temporal responses of the murine microbiota that maintain its capacity for butyrate production correlate with sustained antinociception to chronic morphine.

Gut Microbes. 2025-12

[9]
Cold-pressed extraction of perilla seed oil enriched with alpha-linolenic acid mitigates tumour progression and restores gut microbial homeostasis in the AOM/DSS mice model of colitis-associated colorectal cancer.

PLoS One. 2024-12-9

[10]
Potential of Fiber and Probiotics to Fight Against the Effects of PhIP + DSS-Induced Carcinogenic Process of the Large Intestine.

J Agric Food Chem. 2024-11-13

本文引用的文献

[1]
administration exacerbated the development of colitis-associated colorectal cancer in mice.

J Cancer. 2022-1-1

[2]
Stem cell therapy for Crohn's disease: systematic review and meta-analysis of preclinical and clinical studies.

Stem Cell Res Ther. 2021-8-18

[3]
Suppresses Colorectal Tumorigenesis by Inducing TLR2/NLRP3-Mediated M1-Like TAMs.

Cancer Immunol Res. 2021-10

[4]
The potential of Akkermansia muciniphila in inflammatory bowel disease.

Appl Microbiol Biotechnol. 2021-8

[5]
Interaction between Lipopolysaccharide and Gut Microbiota in Inflammatory Bowel Diseases.

Int J Mol Sci. 2021-6-10

[6]
The Role of Gut Barrier Dysfunction and Microbiome Dysbiosis in Colorectal Cancer Development.

Front Oncol. 2021-4-15

[7]
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.

CA Cancer J Clin. 2021-5

[8]
Progress in Understanding the IL-6/STAT3 Pathway in Colorectal Cancer.

Onco Targets Ther. 2020-12-21

[9]
Use of the synbiotic VSL#3 and yacon-based concentrate attenuates intestinal damage and reduces the abundance of Candidatus Saccharimonas in a colitis-associated carcinogenesis model.

Food Res Int. 2020-11

[10]
The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease.

Microorganisms. 2020-11-1

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索