Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany) http://www.bio.uni-frankfurt.de/48050101.
Institut für Organische und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz, Goethe Universität Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main (Germany).
Angew Chem Int Ed Engl. 2015 Oct 19;54(43):12702-5. doi: 10.1002/anie.201504877. Epub 2015 Aug 25.
Pyrrolizidine alkaloids (PAs) are widespread plant natural products with potent toxicity and bioactivity. Herein, the identification of bacterial PAs from entomopathogenic bacteria using differential analysis by 2D NMR spectroscopy (DANS) and mass spectrometry is described. Their biosynthesis was elucidated to involve a non-ribosomal peptide synthetase. The occurrence of these biosynthesis gene clusters in Gram-negative and Gram-positive bacteria indicates an important biological function in bacteria.
吡咯里西啶生物碱（PAs）是广泛存在于植物中的天然产物，具有很强的毒性和生物活性。本文描述了利用二维核磁共振波谱（DANS）和质谱的差异分析鉴定昆虫病原细菌中的细菌 PAs。研究表明，它们的生物合成涉及一种非核糖体肽合成酶。这些生物合成基因簇在革兰氏阴性菌和革兰氏阳性菌中的存在表明其在细菌中具有重要的生物学功能。
