Department of Bioscience and Biotechnology, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University , 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Korea.
J Agric Food Chem. 2015 Nov 4;63(43):9452-60. doi: 10.1021/acs.jafc.5b03873. Epub 2015 Oct 27.
Non-targeted metabolomic analysis of hot pepper (Capsicum annuum "CM334") was performed at six development stages [16, 25, 36, 38, 43, and 48 days post-anthesis (DPA)] to analyze biochemical changes. Distinct distribution patterns were observed in the changes of metabolites, gene expressions, and antioxidant activities by early (16-25 DPA), breaker (36-38 DPA), and later (43-48 DPA) stages. In the early stages, glycosides of luteolin, apigenin, and quercetin, shikimic acid, γ-aminobutyric acid (GABA), and putrescine were highly distributed but gradually decreased over the breaker stage. At later stages, leucine, isoleucine, proline, phenylalanine, capsaicin, dihydrocapsaicin, and kaempferol glycosides were significantly increased. Pathway analysis revealed metabolite-gene interactions in the biosynthesis of amino acids, capsaicinoids, fatty acid chains, and flavonoids. The changes in antioxidant activity were highly reflective of alterations in metabolites. The present study could provide useful information about nutrient content at each stage of pepper cultivation.
对辣椒（Capsicum annuum“CM334”）在六个发育阶段[16、25、36、38、43 和 48 天授粉后（DPA）]进行非靶向代谢组学分析，以分析生化变化。在早期（16-25 DPA）、破裂期（36-38 DPA）和后期（43-48 DPA）阶段，代谢物、基因表达和抗氧化活性的变化表现出明显的分布模式。在早期阶段，木樨草素、芹菜素和槲皮素的糖苷、莽草酸、γ-氨基丁酸（GABA）和腐胺分布广泛，但在破裂期逐渐减少。在后期阶段，亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、辣椒素、二氢辣椒素和山柰酚糖苷显著增加。途径分析显示了氨基酸、辣椒素类、脂肪酸链和类黄酮生物合成中代谢物-基因的相互作用。抗氧化活性的变化高度反映了代谢物的变化。本研究可为辣椒栽培各阶段的营养成分提供有用信息。
