Kölsch Gregor, Synefiaridou Dimitra
Zoological Institute, Molecular Evolutionary Biology, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany.
Insects. 2012 May 7;3(2):473-91. doi: 10.3390/insects3020473.
When symbioses between insects and bacteria are discussed, the origin of a given association is regularly of interest. We examined the evolution of the symbiosis between reed beetles (Coleoptera, Chrysomelidae, Donaciinae) and intracellular symbionts belonging to the Enterobacteriaceae. We analyzed the partial sequence of the 16S rRNA to assess the phylogenetic relationships with bacteria we found in other beetle groups (Cerambycidae, Anobiidae, other Chrysomelidae). We discuss the ecology of each association in the context of the phylogenetic analysis. The bacteria in Sagra femorata (Chrysomelidae, Sagrinae) are very closely related to those in the Donaciinae and are located in similar mycetomes. The Sagrinae build a cocoon for pupation like the Donaciinae, in which the bacteria produce the material required for the cocoon. These aspects support the close relationship between Sagrinae and Donaciinae derived in earlier studies and make a common ancestry of the symbioses likely. Using PCR primers specific for fungi, we found Candida sp. in the mycetomes of a cerambycid beetle along with the bacteria.
在讨论昆虫与细菌的共生关系时，特定共生关系的起源通常备受关注。我们研究了芦苇甲虫（鞘翅目，叶甲科，水叶甲亚科）与属于肠杆菌科的细胞内共生体之间共生关系的演化。我们分析了16S rRNA的部分序列，以评估与我们在其他甲虫类群（天牛科、窃蠹科、其他叶甲科）中发现的细菌的系统发育关系。我们在系统发育分析的背景下讨论了每种共生关系的生态学。阔胫叶甲（叶甲科，阔胫叶甲亚科）中的细菌与水叶甲亚科中的细菌关系非常密切，且位于相似的菌瘤中。阔胫叶甲亚科像水叶甲亚科一样为化蛹建造茧，细菌在茧中产生所需物质。这些方面支持了早期研究得出的阔胫叶甲亚科与水叶甲亚科之间的密切关系，并使得共生关系有共同起源成为可能。使用针对真菌的特异性PCR引物，我们在一种天牛科甲虫的菌瘤中除细菌外还发现了念珠菌属。
