Smith Richard W, Saroya Rohin, Seymour Colin B, Moccia Richard D, Mothersill Carmel E
a Department of Medical Physics and Applied Radiation Sciences , McMaster University , Hamilton , Ontario, Canada.
b Animal and Poultry Science, University of Guelph , Guelph , Ontario , Canada.
Int J Radiat Biol. 2015;91(11):878-90. doi: 10.3109/09553002.2015.1087065. Epub 2015 Oct 14.
To determine if the legacy of early life stage irradiation seen in rainbow trout is modified in adult fish by acute exposure to waterborne aluminium (Al).
Two-year-old trout which had been irradiated as either eggs 48 h after fertilization, eyed eggs, yolk sac larvae or as first feeders, were exposed to 100 or 200 μg l(-1) Al. These fish were then paired with untreated fish. Gill and skin samples from both the irradiated and Al-exposed, and the untreated bystander fish, were then assayed for the production of toxic or pro-death signals, using the HPV-G reporter cell line.
Depending on the life stage, irradiated and the tissue assayed acute Al exposure modified both the radiation legacy, and the radiation-induced bystander effect in untreated fish, either by reducing reporter cell survival or increasing reporter cell growth.
The effects of early irradiation and adult Al exposure were complex but clearly demonstrated that both the direct radiation effects and the communication of a radiation-induced bystander effect can be modified by a second environmental stressor.
确定虹鳟鱼早期生命阶段辐射的遗留效应在成年鱼中是否会因急性暴露于水中的铝（Al）而改变。
将受精后48小时的鱼卵、眼点卵、卵黄囊幼虫或初次摄食期的鱼进行辐照，两年后将这些鳟鱼暴露于100或200μg l(-1)的铝中。然后将这些鱼与未处理的鱼配对。使用HPV-G报告细胞系，对辐照和暴露于铝的鱼以及未处理的旁观者鱼的鳃和皮肤样本进行检测，以确定是否产生毒性或促死亡信号。
根据生命阶段的不同，辐照鱼和急性铝暴露鱼的组织检测结果表明，急性铝暴露通过降低报告细胞存活率或增加报告细胞生长，改变了辐射遗留效应以及未处理鱼中的辐射诱导旁观者效应。
早期辐照和成年鱼铝暴露的影响很复杂，但清楚地表明，直接辐射效应和辐射诱导旁观者效应的传播都可以被第二种环境应激源改变。
