Neves Mayara A O, Pinheiro Helder H C, Silva Rita S U, Linhares Alexandre C, Silva Luciana D, Gabbay Yvone B, Silva Mônica C M, Loureiro Edvaldo C B, Soares Luana S, Mascarenhas Joana D'Arc P
Center for Biological and Health Sciences, University of State of Pará, Pará, Brazil.
Center for Tropical Medicine, Federal University of Pará, Pará, Brazil.
J Med Virol. 2016 May;88(5):782-9. doi: 10.1002/jmv.24404. Epub 2015 Nov 3.
The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3% (44/241) of the children with acute diarrhea and in 1.2% (3/247) of the non-diarrheic children (P < 0.001), with overall RVA-positivity of 9.6% (47/488). The most common genotype was G2P[4] with 43.2% (19/44) of the diarrheic cases, followed by G12P[8] (27.3%, 12/44), G3P[6] (18.2%, 8/44), G3P[8] (4.5%, 2/44), and G12P[6] (2.3%, 1/44). G12 samples belonged to lineage III and were from children aged 4-52 months. All of these children had acute diarrhea associated with fever (83.3%, 10/12) and vomiting (66.7%, 8/12). Most of the cases occurred in August (58.3%, 7/12), 75% (9/12) of which having received the full vaccination scheme with Rotarix™. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies.
本研究旨在对阿克里州里奥布兰科市轮状病毒疫苗接种后时期的循环轮状病毒（RVA）毒株进行分子特征分析，特别是针对新兴且日益普遍的G12P[8]基因型。2012年1月至12月期间，共收集了488份腹泻和非腹泻儿童的粪便标本。RVA检测最初采用酶联免疫吸附测定（ELISA）方法，随后使用特异性引物进行逆转录聚合酶链反应（RT-PCR）。在患有急性腹泻的儿童中，18.3%（44/241）检测出RVA，在非腹泻儿童中，1.2%（3/247）检测出RVA（P＜0.001），总体RVA阳性率为9.6%（47/488）。最常见的基因型是G2P[4]，在腹泻病例中占43.2%（19/44），其次是G12P[8]（27.3%，12/44）、G3P[6]（18.2%，8/44）、G3P[8]（4.5%，2/44）和G12P[6]（2.3%，1/44）。G12样本属于III型谱系，来自4至52个月大的儿童。所有这些儿童都患有与发热（83.3%，10/12）和呕吐（66.7%，8/12）相关的急性腹泻。大多数病例发生在8月（58.3%，7/12），其中75%（9/12）的儿童已接种完整的Rotarix™疫苗接种方案。G12首次在巴西以相对较高的流行率被报道。我们的研究结果表明，在巴西及其他地区引入轮状病毒疫苗后，有必要对循环RVA毒株的分子特征进行进一步的监测研究，因为不寻常或新兴基因型的出现可能对疫苗接种策略构成挑战。
