Melo A L T, Witter R, Martins T F, Pacheco T A, Alves A S, Chitarra C S, Dutra V, Nakazato L, Pacheco R C, Labruna M B, Aguiar D M
Departamento de Clínica Médica Veterinária, Faculdade de Agronomia, Medicina Veterinária e Zootecnia da Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
Med Vet Entomol. 2016 Mar;30(1):112-6. doi: 10.1111/mve.12139. Epub 2015 Oct 15.
Tick and blood samples collected from domestic dogs in the Brazilian Pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. Among 320 sampled dogs, 3.13% were infected by Babesia vogeli (Piroplasmida: Babesiidae), 8.75% by Hepatozoon canis (Eucoccidiorida: Hepatozoidae), 7.19% by Anaplasma platys (Rickettsiales: Anaplasmataceae), and 0.94% by an unclassified Anaplasma sp. In three tick species collected from dogs, the following tick-borne agents were detected: (a) B. vogeli, An. platys and Ehrlichia canis (Rickettsiales: Anaplasmataceae), infecting Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) ticks; (b) H. canis, an unclassified Anaplasma sp. and Rickettsia amblyommii (Rickettsiales: Rickettsiaceae), infecting Amblyomma cajennense sensu lato (Ixodida: Ixodidae) ticks, and (c) Rickettsia sp. strain Atlantic rainforest, an emerging human pathogen, infecting Amblyomma ovale ticks. Molecular analysis, based on a mitochondrial gene, revealed that the Am. cajennense s.l. ticks of the present study corresponded to Amblyomma sculptum, a member of the Am. cajennense species complex, and that Rh. sanguineus s.l. belonged to the tropical lineage. Whereas dogs are exposed to a number of tick-borne bacterial and protozoan agents in the Pantanal biome, humans are potentially exposed to infection by spotted fever group rickettsiae (e.g. R. amblyommii and Rickettsia sp. strain Atlantic rainforest) because both Am. sculptum and Am. ovale are among the most important human-biting ticks in Brazil.
对从巴西潘塔纳尔湿地的家犬身上采集的蜱虫和血液样本进行了分子方法检测，以确定是否存在蜱传原生动物和细菌。在320只采样犬中，3.13%感染了伯氏巴贝斯虫（梨形虫纲：巴贝斯虫科），8.75%感染了犬肝簇虫（真球虫目：肝簇虫科），7.19%感染了血小板无形体（立克次氏体目：无形体科），0.94%感染了一种未分类的无形体。在从犬身上采集的三种蜱虫中，检测到以下蜱传病原体：(a) 伯氏巴贝斯虫、血小板无形体和犬埃立克体（立克次氏体目：无形体科），感染血红扇头蜱（硬蜱目：硬蜱科）；(b) 犬肝簇虫、一种未分类的无形体和安氏立克次体（立克次氏体目：立克次体科），感染卡延花蜱（硬蜱目：硬蜱科）；(c) 立克次氏体菌株大西洋雨林，一种新出现的人类病原体，感染卵形花蜱。基于线粒体基因的分子分析表明，本研究中的卡延花蜱对应于雕刻花蜱，它是卡延花蜱物种复合体的成员，而血红扇头蜱属于热带谱系。虽然犬在潘塔纳尔生物群落中接触多种蜱传细菌和原生动物病原体，但人类有感染斑点热群立克次氏体（如安氏立克次体和立克次氏体菌株大西洋雨林）的潜在风险，因为雕刻花蜱和卵形花蜱都是巴西最重要的叮咬人类的蜱虫。
