Jia Feiyu, Guo Siyu, Shen Yue, Gao Meiyue, Liu Chongxi, Zhou Shuyu, Li Jiansong, Guan Xuejiao, Wang Xiangjing, Xiang Wensheng
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2016 Jan;109(1):43-50. doi: 10.1007/s10482-015-0608-y. Epub 2015 Oct 14.
Two novel actinobacteria, designated strains NEAU-JM1(T) and NEAU-CL2(T), were isolated from volcanic sediment and the rhizosphere soil of Paris polyphylla, respectively, collected from Jiling and Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibit 99.5 % 16S rRNA gene sequence similarity with each other. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-JM1(T) showed it to be closely related to Catellatospora methionotrophica JCM 7543(T) (99.4 % sequence similarity), Catellatospora coxensis DSM 44901(T) (99.4 %), Catellatospora citrea DSM 44097(T) (99.3 %) and Catellatospora chokoriensis JCM 12950(T) (99.2 %), and that of strain NEAU-CL2(T) to C. citrea DSM 44097(T) (99.4 %), C. methionotrophica JCM 7543(T) (99.3 %), C. chokoriensis JCM 12950(T) (99.3 %) and C. coxensis DSM 44901(T) (99.2 %). However, the DNA-DNA hybridization value between strains NEAU-JM1(T) and NEAU-CL2(T) was 62.2 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-JM1(T) and NEAU-CL2(T) can be concluded to represent two novel species of the genus Catellatospora, for which the names Catellatospora vulcania sp. nov. and Catellatospora paridis sp. nov. are proposed. The type strains are NEAU-JM1(T) (=CGMCC 4.7174(T) = JCM 30054(T)) and NEAU-CL2(T) (=CGMCC 4.7236(T) = DSM 100519(T)), respectively.
分别从中国黑龙江省鸡岭采集的火山沉积物和七叶一枝花根际土壤中分离出两株新型放线菌，分别命名为菌株NEAU-JM1(T)和NEAU-CL2(T)。开展了多相分类研究以确定这些菌株的分类地位。16S rRNA基因序列分析表明，这两株新分离菌株彼此之间的16S rRNA基因序列相似性为99.5%。基于菌株NEAU-JM1(T)的16S rRNA基因序列的系统发育分析表明，它与嗜蛋氨酸卡特拉孢菌JCM 7543(T)（序列相似性99.4%）、考克斯卡特拉孢菌DSM 44901(T)（99.4%）、柠檬卡特拉孢菌DSM 44097(T)（99.3%）和乔科里卡特拉孢菌JCM 12950(T)（99.2%）密切相关，而菌株NEAU-CL2(T)与柠檬卡特拉孢菌DSM 44097(T)（99.4%）、嗜蛋氨酸卡特拉孢菌JCM 7543(T)（99.3%）、乔科里卡特拉孢菌JCM 12950(T)（99.3%）和考克斯卡特拉孢菌DSM 44901(T)（99.2%）密切相关。然而，菌株NEAU-JM1(T)和NEAU-CL2(T)之间的DNA-DNA杂交值为62.2%，这两株菌株与其近缘系统发育亲缘菌株之间的值也低于70%。参照表型特征、系统发育数据和DNA-DNA杂交结果，这两株菌株可彼此区分，也可与它们的近缘系统发育亲缘菌株区分开来。因此，可以得出结论，菌株NEAU-JM1(T)和NEAU-CL2(T)代表卡特拉孢菌属的两个新物种，为此提出新名称火山卡特拉孢菌（Catellatospora vulcania sp. nov.）和七叶一枝花卡特拉孢菌（Catellatospora paridis sp. nov.）。模式菌株分别为NEAU-JM1(T)（=CGMCC 4.7174(T) = JCM 30054(T)）和NEAU-CL2(T)（=CGMCC 4.7236(T) = DSM 100519(T)）。
