Vieira Rúbia da Rocha, Ferreira Lígia Lavezo, Biasoli Éder Ricardo, Bernabé Daniel Galera, Nunes Cáris Maroni, Miyahara Glauco Issamu
Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
Animal Health and Production, Araçatuba School of Veterinary Medicine, Araçatuba, São Paulo, Brazil.
J Clin Pathol. 2016 Apr;69(4):358-63. doi: 10.1136/jclinpath-2015-203325. Epub 2015 Oct 14.
To detect the presence of Epstein-Barr virus (EBV) DNA in different sources of materials from a matched group of patients with oral lichen planus (OLP) and a group of people without OLP lesions, and to correlate the presence of virus with epidemiological variables of the groups studied.
Fresh tissue samples, saliva, exfoliated cells and plasma of 24 patients with OLP lesions (cases) and 17 patients without OLP lesions (controls) were collected. EBV was detected by nested PCR.
Viral positivity was obtained in 62.5% of tissue samples; in 70.8% of exfoliated cell samples; in 33.3% of blood plasma samples and in 75% of saliva samples in the cases; and in 35.3% of tissue samples; 82.4% of exfoliated cell samples; in 47.1% of blood plasma samples and in 64.7% of saliva samples in the controls. There was a predominance of women in both groups. Variables not atrophic-erosive were most affected by EBV.
No relationship between EBV and OLP was found. However, all sources tested in this study were considered suitable for the detection of viruses.
检测一组匹配的口腔扁平苔藓（OLP）患者和一组无OLP病变人群的不同材料来源中是否存在爱泼斯坦-巴尔病毒（EBV）DNA，并将病毒的存在与所研究组的流行病学变量相关联。
收集24例有OLP病变患者（病例组）和17例无OLP病变患者（对照组）的新鲜组织样本、唾液、脱落细胞和血浆。通过巢式PCR检测EBV。
病例组中，62.5%的组织样本、70.8%的脱落细胞样本、33.3%的血浆样本和75%的唾液样本病毒呈阳性；对照组中，35.3%的组织样本、82.4%的脱落细胞样本、47.1%的血浆样本和64.7%的唾液样本病毒呈阳性。两组中女性占多数。非萎缩性糜烂性变量受EBV影响最大。
未发现EBV与OLP之间存在关联。然而，本研究中检测的所有材料来源都被认为适合用于病毒检测。
