Oral Alaeddin, Onel Mustafa, Demirci Mehmet, Baysal Cem, Hulikyan Arat, Uysal Hayriye Kirkoyun, Agacfidan Ali, Ergun Sertan
Department of Oral, Dental and Maxillofacial Surgery, Istanbul University Faculty of Dentistry, Istanbul, Turkiye.
Department of Medical Microbiology, Istanbul University Istanbul Faculty of Medicine, Istanbul, Turkiye.
North Clin Istanb. 2024 Nov 22;11(6):569-574. doi: 10.14744/nci.2024.63239. eCollection 2024.
Oral Lichen Planus (OLP) is an immune system disease and its cause has not been fully determined yet. Oral Lichenoid Contact Lesions (OLCL) is an allergic condition known to develop because of dental materials. It is considered that some infectious agents (e.g., Epstein-Barr virus (EBV)) play roles in the etiology of OLP and OLCL. The purpose of the present study was to investigate the presence of EBV in different clinical samples of patients who had OLP and OLCL, to show its relationship with OLCL, and to determine its role in etiopathogenesis in these patients.
Twenty (20) OLCL, twenty-three (23) OLP, and twenty (20) healthy volunteers who applied to Istanbul University Faculty of Dentistry, Oral and Maxillofacial Surgery were included in the study, regardless of gender. Biopsy samples were taken from patients who had a 5mm punch, including the mucosa containing the lesion along with saliva and blood samples, and all clinical samples were sent to the Department of Medical Microbiology Laboratory under appropriate storage conditions. After the isolation of the DNA from clinical samples, EBV DNA was analyzed on the Light Cycler 480 II device by using Real-time PCR (RT-PCR) tests. The evaluation of the statistical data of the results was made by using the SPSS program.
When the data were evaluated, EBV DNA positivity was detected in 13.04% of the patients who had OLP, 10% of the patients who had OLCL, and 5% of the individuals in the Control Group. In saliva samples, EBV DNA was found positive in 21.74% of individuals with OLP, 15% of individuals with OLCL, and 10% of individuals in the Control Group. In the biopsy samples, EBV DNA was detected positive in 21.74% of the OLP patients, 15% of the OLCL patients, and 10% of the Control Group individuals.
Based on the findings of the present study, no significant differences were observed in the presence of EBV DNA or the quantitative viral load between patients with OLP, OLCL, and the Control Group. However, the quantitative EBV DNA results varied depending on the type of clinical sample selected. We believe that comprehensive studies that will include a larger number of samples must be conducted to determine the role of EBV in OLP and OLCL.
口腔扁平苔藓（OLP）是一种免疫系统疾病，其病因尚未完全明确。口腔苔藓样接触性病变（OLCL）是一种已知因牙科材料引发的过敏性病症。据认为，某些感染因子（如爱泼斯坦 - 巴尔病毒（EBV））在OLP和OLCL的病因学中起作用。本研究的目的是调查患有OLP和OLCL的患者不同临床样本中EBV的存在情况，显示其与OLCL的关系，并确定其在这些患者病因发病机制中的作用。
本研究纳入了20例OLCL患者、23例OLP患者以及20名前往伊斯坦布尔大学牙科学院口腔颌面外科就诊的健康志愿者，不限性别。从患者身上取5毫米钻孔活检样本，包括含有病变的黏膜以及唾液和血液样本，所有临床样本在适当的储存条件下被送往医学微生物学实验室。从临床样本中分离出DNA后，使用实时荧光定量聚合酶链反应（RT-PCR）检测在Light Cycler 480 II设备上分析EBV DNA。使用SPSS程序对结果的统计数据进行评估。
对数据进行评估时，在患有OLP的患者中，13.04%检测到EBV DNA阳性；在患有OLCL的患者中，10%检测到阳性；在对照组个体中，5%检测到阳性。在唾液样本中，OLP患者中有21.74%的个体EBV DNA呈阳性，OLCL患者中有15%，对照组中有10%。在活检样本中，OLP患者中有21.74%检测到EBV DNA阳性，OLCL患者中有15%，对照组中有10%。
基于本研究的结果，在OLP患者、OLCL患者和对照组之间，EBV DNA的存在或病毒载量的定量方面未观察到显著差异。然而，EBV DNA的定量结果因所选临床样本类型而异。我们认为，必须进行包括更多样本的综合研究，以确定EBV在OLP和OLCL中的作用。
