仅用于研究的iCubate iC-GPC检测法在血培养肉汤中鉴定特定革兰氏阳性菌及其耐药决定因素的初步评估
Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths.
作者信息
Buchan Blake W, Reymann Garrett C, Granato Paul A, Alkins Brenda R, Jim Patricia, Young Stephen
机构信息
Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA Wisconsin Diagnostic Laboratory, Milwaukee, Wisconsin, USA
Wisconsin Diagnostic Laboratory, Milwaukee, Wisconsin, USA.
出版信息
J Clin Microbiol. 2015 Dec;53(12):3931-4. doi: 10.1128/JCM.01934-15. Epub 2015 Oct 14.
Abstract
The iC-GPC assay (iCubate, Huntsville, AL) provides a molecular option for the rapid, on-demand analysis of positive blood cultures. A preliminary evaluation of the iC-GPC assay using 203 clinical or seeded specimens demonstrated a sensitivity of 93.8% to 100% and a specificity of 98.0% to 100% for the identification of five Gram-positive bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) and three associated genetic resistance determinants (mecA, vanA, and vanB) in positive blood culture broths.
摘要
iC-GPC检测法（iCubate公司，阿拉巴马州亨茨维尔）为阳性血培养物的快速、按需分析提供了一种分子检测方法。使用203份临床标本或接种标本对iC-GPC检测法进行的初步评估显示，对于阳性血培养肉汤中5种革兰氏阳性菌（金黄色葡萄球菌、表皮葡萄球菌、肺炎链球菌、粪肠球菌和屎肠球菌）以及3种相关耐药基因决定簇（mecA、vanA和vanB）的鉴定，其灵敏度为93.8%至100%，特异性为98.0%至100%。
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