Ugawa Masashi, Lei Cheng, Nozawa Taisuke, Ideguchi Takuro, Di Carlo Dino, Ota Sadao, Ozeki Yasuyuki, Goda Keisuke
Opt Lett. 2015 Oct 15;40(20):4803-6. doi: 10.1364/OL.40.004803.
We present a method for high-throughput optofluidic particle analysis that provides both the morphological and chemical profiles of individual particles in a large heterogeneous population. This method is based on an integration of a time-stretch optical microscope with a submicrometer spatial resolution of 780 nm and a three-color fluorescence analyzer on top of an inertial-focusing microfluidic device. The integrated system can perform image- and fluorescence-based screening of particles with a high throughput of 10,000 particles/s, exceeding previously demonstrated imaging particle analyzers in terms of specificity without sacrificing throughput.
我们提出了一种用于高通量光流粒子分析的方法，该方法能够提供大量异质群体中单个粒子的形态和化学特征。此方法基于将具有780 nm亚微米空间分辨率的时间拉伸光学显微镜与三色荧光分析仪集成在惯性聚焦微流控装置之上。该集成系统能够以每秒10,000个粒子的高通量对粒子进行基于图像和荧光的筛选，在不牺牲通量的情况下，其特异性超过了先前展示的成像粒子分析仪。
