Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
Methods. 2018 Mar 1;136:116-125. doi: 10.1016/j.ymeth.2017.10.004. Epub 2017 Oct 12.
Innovations in optical microscopy have opened new windows onto scientific research, industrial quality control, and medical practice over the last few decades. One of such innovations is optofluidic time-stretch quantitative phase microscopy - an emerging method for high-throughput quantitative phase imaging that builds on the interference between temporally stretched signal and reference pulses by using dispersive properties of light in both spatial and temporal domains in an interferometric configuration on a microfluidic platform. It achieves the continuous acquisition of both intensity and phase images with a high throughput of more than 10,000 particles or cells per second by overcoming speed limitations that exist in conventional quantitative phase imaging methods. Applications enabled by such capabilities are versatile and include characterization of cancer cells and microalgal cultures. In this paper, we review the principles and applications of optofluidic time-stretch quantitative phase microscopy and discuss its future perspective.
在过去的几十年中，光学显微镜的创新为科学研究、工业质量控制和医学实践开辟了新的窗口。其中一项创新是光流体时拉伸定量相显微镜——这是一种新兴的高通量定量相成像方法，它利用微流控平台上的干涉配置中光在空间和时间域中的色散特性，在时间上拉伸的信号和参考脉冲之间的干涉。它通过克服传统定量相成像方法中存在的速度限制，以每秒超过 10000 个颗粒或细胞的高通量实现了强度和相位图像的连续采集。这种能力实现的应用非常多样化，包括癌症细胞和微藻培养物的特征描述。本文综述了光流体时拉伸定量相显微镜的原理和应用，并讨论了其未来展望。
