Chinnakkannu Vijayakumar Chandramouli, Venkatakrishnan Krishnan, Tan Bo
Ultrashort laser nano manufacturing research facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, Canada, M5B 2K3.
Nano imaging lab, Department of Aerospace Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, Canada, M5B 2K3.
Sci Rep. 2015 Oct 15;5:15294. doi: 10.1038/srep15294.
Knowledge about cancer cell behavior on heterogeneous nanostructures is relevant for developing a distinct biomaterial that can actuate cancer cells. In this manuscript, we have demonstrated a harmonized approach of forming multi Ti-oxide phases in a nanostructure (MTOP nanostructure) for its unique cancer cell controlling behavior.Conventionally, single phases of TiO2 are used for targeted therapy and as drug carrier systems.In this research, we have shown a biomaterial that can control HeLa cells diligently using a combination of TiO, Ti3O and TiO2 phases when compared to fibroblast (NIH3T3) cells.MTOP-nanostructures are generated by varying the ionization energy in the vapor plume of the ultrashort pulse laser; this interaction with the material allows accurate tuning and composition of phases within the nanostructure. In addition, the lattice spacing of MTOP-nanostructures was analyzed as shown by HR-TEM investigations. An FESEM investigation of MTOP-nanostructures revealed a greater reduction of HeLa cells relative to fibroblast cells. Altered cell adhesion was followed by modulation of HeLa cell architecture with a significant reduction of actin stress fibers.The intricate combination of MTOP-nanostructures renders a biomaterial that can precisely alter HeLa cell but not fibroblast cell behavior, filling a void in the research for a biomaterial to modulate cancer cell behavior.
了解癌细胞在异质纳米结构上的行为对于开发一种能够驱动癌细胞的独特生物材料具有重要意义。在本论文中，我们展示了一种在纳米结构中形成多氧化钛相（MTOP纳米结构）的协调方法，因其具有独特的癌细胞控制行为。传统上，二氧化钛的单相用于靶向治疗和作为药物载体系统。在本研究中，我们展示了一种生物材料，与成纤维细胞（NIH3T3）相比，它可以通过TiO、Ti3O和TiO2相的组合有效地控制HeLa细胞。MTOP纳米结构是通过改变超短脉冲激光蒸汽羽流中的电离能产生的；这种与材料的相互作用允许对纳米结构内的相进行精确调整和组成。此外，如高分辨率透射电子显微镜（HR-TEM）研究所示，对MTOP纳米结构的晶格间距进行了分析。对MTOP纳米结构的场发射扫描电子显微镜（FESEM）研究表明，相对于成纤维细胞，HeLa细胞的减少更为显著。细胞粘附的改变之后是HeLa细胞结构的调节，肌动蛋白应力纤维显著减少。MTOP纳米结构的复杂组合产生了一种生物材料，它可以精确改变HeLa细胞的行为，但不能改变成纤维细胞的行为，填补了调节癌细胞行为的生物材料研究中的空白。
