Kongkapan Jutamart, Poapolathep Saranya, Isariyodom Supaporn, Kumagai Susumu, Poapolathep Amnart
Interdisciplinary Graduate Program in Agricultural Biotechnology, Graduate School, Kasetsart University, KamphaengSaen Campus, Nakhon, Pathom 73140, Thailand.
J Vet Med Sci. 2016 Feb;78(2):259-64. doi: 10.1292/jvms.15-0317. Epub 2015 Oct 16.
Mycotoxins are secondary fungal metabolites that are typically present in grain and feed ingredients used for animal feeds. An analytical method using LC-ESI-MS/MS was developed to quantify nine mycotoxins, consisting of aflatoxin B1 (AFB1), AFB2, AFG1, AFG2, T-2 toxin, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and ochratoxin A (OTA) in broiler feeds. In total, 100 samples of broiler feeds were collected from poultry farms in Central Thailand. The survey found that AFB1 and ZEA were the most prevalent mycotoxins in the feed samples at percentages of 93% and 63%, respectively. The limit of detections (LODs) of investigated mycotoxins was 0.20-0.78 ng/g. AFB2, DON, AFG1, NIV and T-2 toxin were also detectable at low contamination levels with percentages of 20%, 9%, 7%, 5% and 1%, respectively, whereas OTA and AFG2 were not detected in any of the feed samples. These results suggest that there is a very low level of risk of the exposure to mycotoxins in feeds obtained from broiler farms in Central Thailand.
霉菌毒素是真菌的次生代谢产物，通常存在于用于动物饲料的谷物和饲料原料中。开发了一种使用液相色谱 - 电喷雾串联质谱法（LC - ESI - MS/MS）的分析方法，用于定量测定肉鸡饲料中的九种霉菌毒素，包括黄曲霉毒素B1（AFB1）、AFB2、AFG1、AFG2、T - 2毒素、脱氧雪腐镰刀菌烯醇（DON）、雪腐镰刀菌烯醇（NIV）、玉米赤霉烯酮（ZEA）和赭曲霉毒素A（OTA）。总共从泰国中部的家禽养殖场收集了100份肉鸡饲料样本。调查发现，AFB1和ZEA是饲料样本中最普遍的霉菌毒素，分别占93%和63%。所研究霉菌毒素的检测限（LOD）为0.20 - 0.78 ng/g。AFB2、DON、AFG1、NIV和T - 2毒素在低污染水平下也可检测到，分别占20%、9%、7%、5%和1%，而在任何饲料样本中均未检测到OTA和AFG2。这些结果表明，从泰国中部肉鸡养殖场获得的饲料中，霉菌毒素暴露风险非常低。
