用于骨科应用的多孔Ti6Al4V/壳聚糖海绵复合支架的研发。
Development of porous Ti6Al4V/chitosan sponge composite scaffold for orthopedic applications.
作者信息
Guo Miao, Li Xiang
机构信息
College of Life Information Science & Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
School of Mechanical Engineering, Shanghai Jiao Tong University, State Key Laboratory of Mechanical System and Vibration, Shanghai 200240, China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2016 Jan 1;58:1177-81. doi: 10.1016/j.msec.2015.09.061. Epub 2015 Sep 15.
A novel composite scaffold consisting of porous Ti6Al4V part filled with chitosan sponge was fabricated using a combination of electron beam melting and freeze-drying. The mechanical properties of porous Ti6Al4V part were examined via compressive test. The ultimate compressive strength was 85.35 ± 8.68 MPa and the compressive modulus was 2.26 ± 0.42 GPa. The microstructure of composite scaffold was characterized using scanning electron microscopy. The chitosan sponge filled in Ti6Al4V part exhibited highly porous and well-interconnected micro-pore architecture. The osteoblastic cells were seeded on scaffolds to test their seeding efficiency and biocompatibility. Significantly higher cell seeding efficiency was found on composite scaffold. The biological response of osteoblasts on composite scaffolds was superior in terms of improved cell attachment, higher proliferation, and well-spread morphology in relation to porous Ti6Al4V part. These results suggest that the Ti6Al4V/chitosan composite scaffold is potentially useful as a biomedical scaffold for orthopedic applications.
通过电子束熔化和冷冻干燥相结合的方法，制备了一种新型复合支架，该支架由填充壳聚糖海绵的多孔Ti6Al4V部分组成。通过压缩试验检测多孔Ti6Al4V部分的力学性能。极限抗压强度为85.35±8.68MPa，压缩模量为2.26±0.42GPa。使用扫描电子显微镜对复合支架的微观结构进行表征。填充在Ti6Al4V部分的壳聚糖海绵呈现出高度多孔且相互连通良好的微孔结构。将成骨细胞接种在支架上以测试其接种效率和生物相容性。在复合支架上发现细胞接种效率显著更高。与多孔Ti6Al4V部分相比，成骨细胞在复合支架上的生物学反应在改善细胞附着、更高的增殖和良好伸展的形态方面更优越。这些结果表明，Ti6Al4V/壳聚糖复合支架作为骨科应用的生物医学支架具有潜在用途。
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