就诊于生育诊所的男性患者小容量血清样本中二噁英类多氯联苯的水平。
Levels of dioxin-like PCBs in low-volume serum samples of male patients attending fertility clinics.
作者信息
Moltó Julia, Paul Raiza, Ortuño Nuria, Medrano María Llanos, Aizpurua Jon, Gómez-Torres María José
机构信息
Chemical Engineering Department, University of Alicante, Carretera de San Vicente s/n, P.O. Box 99, 03080, Alicante, Spain.
Biotechnology Department, University of Alicante, Carretera de San Vicente s/n, P.O. Box 99, 03080, Alicante, Spain.
出版信息
Environ Sci Pollut Res Int. 2016 Feb;23(4):3463-8. doi: 10.1007/s11356-015-5571-7. Epub 2015 Oct 21.
An accurate and easy method for the extraction, cleanup, and HRGC-HRMS analysis of dioxin-like PCBs (DL-PCBs) in low-volume serum samples (1 mL) was developed. Serum samples were extracted several times using n-hexane and purified by acid washing. Recovery rates of labeled congeners ranged from 70 to 110% and the limits of detection were below 1 pg/g on lipid basis. Although human studies are limited and contradictory, several studies have shown that DL-PCBs can have adverse effects on the male reproductive system. In this way, the present method was applied to 21 serum samples of male patients attending fertility clinics. The total levels obtained for the patients ranged from 6.90 to 84.1 pg WHO-TEQ/g lipid, with a mean value of 20.3 pg WHO-TEQ/g lipid. The predominant PCBs (the sum of PCB 118, 156, and 105) contributed 67% to the mean concentration of total DL-PCBs in the samples analyzed.
开发了一种准确、简便的方法，用于低体积血清样本（1 mL）中二噁英类多氯联苯（DL-PCBs）的提取、净化及高分辨气相色谱-高分辨质谱（HRGC-HRMS）分析。血清样本用正己烷多次萃取，并用酸洗净化。标记同系物的回收率在70%至110%之间，基于脂质的检测限低于1 pg/g。尽管人体研究有限且相互矛盾，但多项研究表明，DL-PCBs可对男性生殖系统产生不良影响。通过这种方式，本方法应用于21例前往生育诊所就诊的男性患者的血清样本。患者的总水平在6.90至84.1 pg WHO-TEQ/g脂质之间，平均值为20.3 pg WHO-TEQ/g脂质。主要的多氯联苯（PCB 118、156和105的总和）占所分析样本中总DL-PCBs平均浓度的67%。
Zotero 插件