Cok Ismet, Donmez Menekse Keski, Satiroğlu M Hakan, Aydinuraz Batu, Henkelmann Bernhard, Shen Heqing, Kotalik Jarmila, Schramm Karl-Werner
Department of Toxicology, Faculty of Pharmacy, Gazi University, 06330, Hipodrom, Ankara, Turkey.
Arch Environ Contam Toxicol. 2008 Jul;55(1):143-52. doi: 10.1007/s00244-007-9094-1. Epub 2008 Jan 1.
Some epidemiological studies suggested the occurrence of an alteration in the male reproductive function in the past 50 years, particularly a decrease in the sperm count and quality, an increase in the malformations frequency of the reproductive apparatus (cryptorchidism and hypospadias) and of testicular cancers. Especially according the laboratory animals studies, polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) have been suspected to play a crucial and deleterious role in the alteration of human fertility. For this reason, we measured adipose tissue concentrations of PCDDs/Fs and dioxin-like PCBs in 23 fertile and 22 infertile men living in Ankara, Turkey. Adipose tissue samples were analyzed for PCDD/F and 12 dioxin-like PCB congeners using high-resolution gas chromatography/high-resolution mass spectrometry. For the fertile and infertile groups, the World Health Organization (WHO)(PCDD/F)-TEQ concentrations ranged from 3.0 to 15.8 pg/g fat and from 2.8 to 17.2 pg/g fat, respectively (4.4-31.5 and 4.7-22.3 WHO-TEQs/g fat, respectively, including dioxin-like PCBs) (p > 0.05). The mean concentrations of WHO(PCDD/F)-TEQ and WHO(PCB)-TEQ have been calculated as 7.2 and 12.5 pg/g (on a lipid basis) for the fertile group and 7.0 and 9.4 pg/g for the infertile group, respectively. Concentrations of each of the PCDD/F and dioxin-like PCB congeners were compared in fertile and infertile groups among themselves, and no statistical significance was obtained (p > 0.05), except 2,3,7,8-tetrachlorodibenzofuran (p = 0.0029) and 1,2,3,4,6,7,8,9-octachlorodibenzofuran (p = 0.01).
一些流行病学研究表明，在过去50年里男性生殖功能出现了变化，尤其是精子数量和质量下降，生殖器官（隐睾症和尿道下裂）畸形频率以及睾丸癌发病率增加。特别是根据实验动物研究，多氯联苯（PCBs）、多氯二苯并对二恶英（PCDDs）和多氯二苯并呋喃（PCDFs）被怀疑在人类生育能力改变中起关键的有害作用。因此，我们测量了居住在土耳其安卡拉的23名有生育能力男性和22名不育男性脂肪组织中PCDDs/Fs和类二恶英多氯联苯的浓度。使用高分辨率气相色谱/高分辨率质谱分析脂肪组织样本中的PCDD/F和12种类二恶英多氯联苯同系物。对于有生育能力组和不育组，世界卫生组织（WHO）（PCDD/F）-毒性当量浓度分别为3.0至15.8 pg/g脂肪和2.8至17.2 pg/g脂肪（分别为4.4 - 31.5和4.7 - 22.3 WHO-毒性当量/g脂肪，包括类二恶英多氯联苯）（p>0.05）。有生育能力组的WHO（PCDD/F）-毒性当量和WHO（PCB）-毒性当量的平均浓度分别计算为7.2和12.5 pg/g（基于脂质），不育组分别为7.0和9.4 pg/g。比较了有生育能力组和不育组中PCDD/F和类二恶英多氯联苯同系物各自的浓度，除了2,3,7,8-四氯二苯并呋喃（p = 0.0029）和1,2,3,4,6,7,8,9-八氯二苯并呋喃（p = 0.01）外，未获得统计学意义（p>0.05）。
