对HIV-1免疫印迹结果存疑的献血者进行HIV-2抗体检测。

HIV-2 antibody testing of blood donors with doubtful immunoblot results for HIV-1.

作者信息

Knüver-Hopf J, Mohr H, Beyer J, Lambrecht B, Mix D, Rübsamen-Waigmann H, Schmitt H

机构信息

German Red Cross Blood Transfusion Service of Lower Saxony, Springe, Federal Republic of Germany.

出版信息

Blut. 1989 Mar;58(3):155-7. doi: 10.1007/BF00320437.

DOI:10.1007/BF00320437

PMID:2649189

Abstract

Antibodies against human immunodeficiency virus type-1 (HIV-1) in samples from blood donors are commonly detected by various enzyme-linked immunosorbent assays (ELISA) and by confirmatory tests, e.g., "Western blot" or immunofluorescence tests. Immunoblot reactivity, which is directed only towards the HIV-1 core proteins p 18, p 24 and p 55, may represent false-positive reactions. Out of 125,000 blood donations, 140 were repeatably HIV-1 antibody reactive by ELISA; of these, 20 were doubtful positive sera with isolated p 18 and/or p 24 bands in the HIV-1 confirmatory assay. Antibodies to HIV-2 are known to cross-react with these HIV-1 core proteins. We therefore assayed the 20 sera by immunofluorescence and immunoblotting for the presence of antibodies to HIV-2. None of these doubtful HIV-1 antibody positive blood donor sera was found to have antibodies to HIV-2.

摘要

通常通过各种酶联免疫吸附测定（ELISA）以及确证试验，例如“免疫印迹法”或免疫荧光试验，来检测献血者样本中针对1型人类免疫缺陷病毒（HIV-1）的抗体。仅针对HIV-1核心蛋白p18、p24和p55的免疫印迹反应性可能代表假阳性反应。在125,000份献血样本中，有140份通过ELISA反复检测出HIV-1抗体呈反应性；其中，有20份为可疑阳性血清，在HIV-1确证试验中出现孤立的p18和/或p24条带。已知针对HIV-2的抗体可与这些HIV-1核心蛋白发生交叉反应。因此，我们通过免疫荧光和免疫印迹法对这20份血清进行检测，以确定是否存在针对HIV-2的抗体。这些可疑的HIV-1抗体阳性献血者血清中均未发现针对HIV-2的抗体。