Crystallization and preliminary X-ray analysis of the human c-H-ras-oncogene product p21 complexed with GTP analogues.

作者信息

Scherer A, John J, Linke R, Goody R S, Wittinghofer A, Pai E F, Homes K C

机构信息

Biophysik, Max-Planck-Institut für Medizinische Forschung, Heidelberg, F.R.G.

出版信息

J Mol Biol. 1989 Mar 5;206(1):257-9. doi: 10.1016/0022-2836(89)90540-8.

The catalytic domain (amino acid residues 1 to 166) of the human ras-oncogene product p21 complexed with the GTP analogues beta,gamma-imido-GTP (GMPPNP), beta,gamma-methylene-GTP (GMPPCP), and guanosine-5'-(gamma-thiotriphosphate) (GTP gamma S) have been been crystallized. Crystals of the GMPPNP and GMPPCP complexes are well suited for high resolution X-ray crystallography. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) with unit cell axes a=b=40.3 A and c = 162.2 A.