Katoozi Delaram, Clayton Andrew H A, Moss David J, Chon James W M
Optical Sciences Centre, Department of Physics and Astronomy, Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Victoria, Australia.
Biomed Opt Express. 2020 Dec 22;12(1):539-552. doi: 10.1364/BOE.417321. eCollection 2021 Jan 1.
The application of gold nanoparticles (AuNPs) in cancer therapeutics and diagnostics has recently reached a clinical level. Functional use of the AuNP in theranostics first requires effective uptake into the cells, but accurate quantification of AuNPs cellular uptake in real-time is still a challenge due to the destructive nature of existing characterization methods. The optical imaging-based quantification method is highly desirable. Here, we propose the use of high-order image correlation spectroscopy (HICS) as an optical imaging-based nanoparticle quantification technique. Coupled with dark field microscopy (DFM), a non-destructive and easy quantification method could be achieved. We demonstrate HICS analysis on 80 nm AuNPs coated with cetyltrimethylammonium bromide (CTAB) uptake in HeLa cells to calculate the percentage of aggregate species (dimer) in the total uptake and their relative scattering quantum yield inside the cells, the details of which are not available with other quantification techniques. The total particle uptake kinetics measured were in a reasonable agreement with the literature.
金纳米颗粒（AuNPs）在癌症治疗和诊断中的应用最近已达到临床水平。AuNP在治疗诊断学中的功能应用首先需要有效地摄取到细胞中，但由于现有表征方法具有破坏性，实时准确量化AuNPs的细胞摄取仍然是一个挑战。基于光学成像的量化方法非常可取。在此，我们提出使用高阶图像相关光谱（HICS）作为基于光学成像的纳米颗粒量化技术。结合暗场显微镜（DFM），可以实现一种无损且易于量化的方法。我们展示了对涂有十六烷基三甲基溴化铵（CTAB）的80 nm AuNPs在HeLa细胞中的摄取进行HICS分析，以计算总摄取中聚集体物种（二聚体）的百分比及其在细胞内的相对散射量子产率，而其他量化技术无法获得这些细节。所测量的总颗粒摄取动力学与文献中的结果合理一致。
