Yang Hye Jeong, Ko Byung-Seob, Kwon Dae Young, Lee Hye Won, Kim Min Jung, Ryuk Jina, Kang Suna, Kim Da Sol, Park Sunmin
1Division of Metabolism and Functionality Research, Korean Food Research Institutes, Sungnam 2Korea Institute of Oriental Medicine, Daejeon 3Department of Food and Nutrition, Obesity/Diabetes Research Center, Hoseo University, Asan, South Korea.
Menopause. 2016 Feb;23(2):197-208. doi: 10.1097/GME.0000000000000521.
We investigated whether long-term consumption of Korean mistletoe or Asian Ulmi cortex would prevent or delay menopausal symptoms and progression of osteoarthritis in estrogen-deficient obese rats.
Ovariectomized (OVX) rats were provided a 45% fat diet containing either (1) 0.6% lyophilized water extract of Korean mistletoe (KME) + 1.4% dextrose (KME; n = 10), (2) 2% lyophilized water extract of Ulmi cortex (UCE; n = 10), (3) 30 μg/kg bw 17β-estradiol + 2% dextrose (positive control; n = 10), (4) 2% dextrose (placebo; OVX-control; n = 10), or (5) 2% dextrose (normal-control; n = 10) for 4 weeks. At the beginning of the 5th week, OVX rats, except in the normal-control group, were given articular injections of monoiodoacetate into the right knee and the assigned diets were provided for an additional 3 weeks. The rats in the normal-control had injections of saline into the right knee.
KME, but not UCE, partially prevented the insulin resistance and the loss of bone mineral density and lean mass. The limping scores were lower in the descending order of the OVX-control > KME and 17β-estradiol > UCE > normal-control at day 14 and 21 (P < 0.05). The scores for pain behaviors measured by weight distribution on the right leg, maximum running velocity on a treadmill and locomotive activity, were markedly decreased in the same order as limping scores. Monoiodoacetate increased the expression of matrix metalloprotinase-3 and metalloprotinase-13 in the articular cartilage and elevated the production of inflammatory markers such as tumor necrosis factor-α, interleukin-1β, and interleukin-6, but they were lower in the UCE than in the other groups (P < 0.05). Histology of the right knee revealed cartilage damage near the tidemark of the knee and proteoglycan loss was markedly less in UCE.
UCE was an effective therapeutic agent for preventing osteoarthritis and KME prevented decreases in lean body mass, bone mineral density, and insulin sensitivity in estrogen-deficient rats.
我们研究了长期食用韩国槲寄生或杜仲皮是否能预防或延缓雌激素缺乏的肥胖大鼠的更年期症状和骨关节炎进展。
给去卵巢（OVX）大鼠提供含45%脂肪的饮食，其中分别含有（1）0.6%韩国槲寄生冻干水提取物（KME）+1.4%葡萄糖（KME组；n = 10），（2）2%杜仲皮冻干水提取物（UCE组；n = 10），（3）30 μg/kg体重17β-雌二醇+2%葡萄糖（阳性对照组；n = 10），（4）2%葡萄糖（安慰剂组；OVX-对照组；n = 10），或（5）2%葡萄糖（正常对照组；n = 10），持续4周。在第5周开始时，除正常对照组外，给OVX大鼠右膝关节注射单碘乙酸盐，并继续给予指定饮食3周。正常对照组大鼠右膝关节注射生理盐水。
KME而非UCE能部分预防胰岛素抵抗以及骨矿物质密度和瘦体重的丢失。在第14天和21天，跛行评分从高到低依次为OVX-对照组>KME组和17β-雌二醇组>UCE组>正常对照组（P < 0.05）。通过右腿重量分布、跑步机上的最大跑步速度和运动活动测量的疼痛行为评分，其降低顺序与跛行评分相同。单碘乙酸盐增加了关节软骨中基质金属蛋白酶-3和金属蛋白酶-13的表达，并提高了肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6等炎症标志物的产生，但UCE组中的这些指标低于其他组（P < 0.05）。右膝关节组织学检查显示，靠近膝关节潮标的软骨损伤，且UCE组中蛋白聚糖的丢失明显较少。
UCE是预防骨关节炎的有效治疗剂，KME可预防雌激素缺乏大鼠瘦体重、骨矿物质密度和胰岛素敏感性的降低。
