Park Sunmin, Moon Bo Reum, Kim Ji Eun, Kim Hyun Joo, Zhang Ting
Department Food & Nutrition, Obesity/Diabetes Center, Hoseo University, Asan 31499, Korea.
Department of Bio-Convergence System, Hoseo University, Asan 31499, Korea.
Pharmaceutics. 2020 Dec 21;12(12):1245. doi: 10.3390/pharmaceutics12121245.
Water extracts of both L. root bark (MBW) and Siebold and Zucc fruit (CFW) have traditionally been used to promote men's health in the elderly in Asia. We determined that the 12-week consumption of MBW and CFW could alleviate testosterone-deficiency syndrome and osteoarthritis (OA) symptoms in testosterone-deficient rats, and the action mechanisms were explored. Rats with bilateral orchiectomy (ORX) were fed a 45% fat diet containing either 0.5% MBW (ORX-MBW), 0.5% CFW(ORX-CFW), or 0.5% dextrin (ORX-CON). Sham-operated rats also received 0.5% dextrin (Non-ORX-CON). After 8 weeks of treatment, all rats had an injection of monoiodoacetate (MIA) into the left knee, and they continued the same diet for the additional 4 weeks. ORX-CFW and ORX-MBW partially prevented the reduction of serum testosterone concentrations and decreased insulin resistance, compared to the ORX-CON. ORX-CFW and ORX-MBW protected against the reduction of bone mineral density (BMD) and lean body mass (LBM) compared to the ORX-CON. The limping and edema scores were lower in the order of the ORX-CON, ORX-CRF = ORX-MBW, and Non-ORX-CON ( < 0.05). The scores for pain behaviors, measured by weight-distribution on the OA leg and maximum running velocity on a treadmill, significantly decreased in the same order as limping scores. ORX-MBW protected against the increased expression of matrix metalloproteinase (MMP)-3 and MMP-13 and reduced the production of inflammatory markers such as TNF-α and IL-1β, by MIA in the articular cartilage, compared to the ORX-CON ( < 0.05). The cartilage damage near the tidemark of the knee and proteoglycan loss was significantly less in ORX-MBW than ORX-CON. In conclusion, MBW, possibly CFW, could be effective alternative therapeutic agents for preventing osteoarthritis in testosterone-deficient elderly men.
在亚洲，光叶楮根皮（MBW）和枳椇果实（CFW）的水提取物传统上一直被用于促进老年人的男性健康。我们确定，连续12周食用MBW和CFW可缓解睾酮缺乏大鼠的睾酮缺乏综合征和骨关节炎（OA）症状，并对其作用机制进行了探索。对双侧睾丸切除（ORX）的大鼠喂食含0.5% MBW（ORX-MBW）、0.5% CFW（ORX-CFW）或0.5%糊精（ORX-CON）的45%脂肪饮食。假手术大鼠也接受0.5%糊精（非ORX-CON）。治疗8周后，所有大鼠左膝注射单碘乙酸盐（MIA），并继续相同饮食4周。与ORX-CON相比，ORX-CFW和ORX-MBW部分预防了血清睾酮浓度的降低，并降低了胰岛素抵抗。与ORX-CON相比，ORX-CFW和ORX-MBW防止了骨矿物质密度（BMD）和去脂体重（LBM）的降低。跛行和水肿评分按ORX-CON、ORX-CRF = ORX-MBW和非ORX-CON顺序降低（<0.05）。通过OA腿上的重量分布和跑步机上的最大跑步速度测量的疼痛行为评分，与跛行评分顺序相同显著降低。与ORX-CON相比，ORX-MBW可防止关节软骨中MIA诱导基质金属蛋白酶（MMP）-3和MMP-13表达增加，并减少炎症标志物如TNF-α和IL-1β的产生（<0.05）。ORX-MBW组膝关节潮线附近软骨损伤和蛋白聚糖损失明显少于ORX-CON组。总之，MBW，可能还有CFW，可能是预防睾酮缺乏老年男性骨关节炎的有效替代治疗药物。
