Zemlianskykh N G, Babijchuk L A
Fiziol Zh (1994). 2016;62(2):94-102. doi: 10.15407/fz62.02.094.
We studied the changes in surface marker CD44 in erythrocytes, cryopreserved under the protection of glycerol and PEG–1500, or stored in hypothermic conditions. It was shown that during hypothermic storage the CD44 characteristics in erythrocyte suspension were unchanged within 10 days. In cryopreserved erythrocytes a reduction in CD44–positive cells and in the level of expression of the surface marker were marked. Using PEG–1500 resulted in more pronounced change in erythrocyte CD44 characteristics after freeze–thawing in comparison with glycerol. Removal of cryoprotectants and the loss of a part of cells during the washing process led to the restoration of the CD44 characteristics in freeze–thawed erythrocytes suspension which successfully survived after the stresses. The results indicate that revealed changes in cryopreserved erythrocytes cover only a part of the cells, and they are associated with the instability of the population of erythrocytes with altered CD44 characteristics wherethrough after the removal of cryoprotectants with concomitant hemolysis of unstable cells the CD44 parameters in erythrocyte suspensions recovered. The mechanisms underlying the changes in the parameters of the surface marker CD44 in freeze–thawed erythrocyte may be related to the disruption of intermolecular interactions in the membrane under the influence of physical and chemical environmental factors, followed by the membrane vesiculation with the inclusion of the CD44 into the vesicles.
我们研究了在甘油和聚乙二醇-1500（PEG–1500）保护下冷冻保存或在低温条件下储存的红细胞表面标志物CD44的变化。结果表明，在低温储存期间，红细胞悬液中的CD44特性在10天内未发生变化。在冷冻保存的红细胞中，CD44阳性细胞数量和表面标志物的表达水平均显著降低。与甘油相比，使用PEG–1500导致冻融后红细胞CD44特性发生更明显的变化。去除冷冻保护剂以及在洗涤过程中部分细胞的损失导致冻融红细胞悬液中CD44特性得以恢复，这些红细胞在经历应激后成功存活。结果表明，冷冻保存红细胞中所揭示的变化仅涉及部分细胞，并且它们与具有改变的CD44特性的红细胞群体的不稳定性有关，由此在去除冷冻保护剂并伴随不稳定细胞溶血后，红细胞悬液中的CD44参数得以恢复。冻融红细胞表面标志物CD44参数变化的潜在机制可能与物理和化学环境因素影响下膜内分子间相互作用的破坏有关，随后膜形成囊泡并将CD44纳入囊泡中。
