Medrano Guillermo, Hermosillo-Rodriguez Jesus, Pham Thuy, Granillo Alejandro, Hartley Craig J, Reddy Anilkumar, Osuna Patricia Mejia, Entman Mark L, Taffet George E
Sections of Cardiovascular Sciences and Geriatrics, Department of Medicine and Huffington Center on Aging, Baylor College of Medicine, and DeBakey Heart Center and Department of Medicine, Houston Methodist Hospital, Houston, Texas.
J Gerontol A Biol Sci Med Sci. 2016 Sep;71(9):1141-50. doi: 10.1093/gerona/glv143. Epub 2015 Oct 28.
Impaired cardiac diastolic function occurs with aging in many species and may be difficult to measure noninvasively. In humans, left atrial (LA) volume is a robust measure of chronic diastolic function as the LA is exposed to increased left ventricular filling pressures. We hypothesized that LA volume would be a useful indicator of diastolic function in aging mice. Further, we asked whether pressures were propagated backwards affecting pulmonary arteries (PAs) and right ventricle (RV). We measured LA, PA, and RV infundibulum dimensions with echocardiography and used mouse-specific Doppler systems and pressure catheters for noninvasive and invasive measures. As C57BL/6 mice aged from 3 to 29-31 months, LA volume almost tripled. LA volume increases correlated with traditional diastolic function measures. Within groups of 14- and 31-month-old mice, LA volume correlated with diastolic function measured invasively. In serial studies, mice evaluated at 20 and 24 months showed monotonic increases in LA volume; other parameters changed less predictably. PA diameters, larger in 30-month-old mice than 6-month-old mice, correlated with LA volumes. Noninvasive LA volume and PA diameter assessments are useful and state independent measures of diastolic function in mice, correlating with other measures of diastolic dysfunction in aging. Furthermore, serial measurements over 4 months demonstrated consistent increases in LA volume suitable for longitudinal cardiac aging studies.
在许多物种中，心脏舒张功能受损会随着年龄增长而出现，并且可能难以通过非侵入性方法进行测量。在人类中，左心房（LA）容积是慢性舒张功能的可靠指标，因为左心房会承受升高的左心室充盈压。我们假设LA容积将是衰老小鼠舒张功能的一个有用指标。此外，我们还研究了压力是否会逆向传导影响肺动脉（PA）和右心室（RV）。我们使用超声心动图测量LA、PA和RV漏斗部的尺寸，并使用针对小鼠的多普勒系统和压力导管进行非侵入性和侵入性测量。随着C57BL/6小鼠从3个月龄长到29 - 31个月龄，LA容积几乎增加了两倍。LA容积的增加与传统的舒张功能指标相关。在14月龄和31月龄小鼠组中，LA容积与侵入性测量的舒张功能相关。在系列研究中，在20个月龄和24个月龄评估的小鼠显示LA容积呈单调增加；其他参数的变化则较难预测。30月龄小鼠的PA直径大于6月龄小鼠，且与LA容积相关。非侵入性的LA容积和PA直径评估是小鼠舒张功能的有用且与状态无关的测量指标，与衰老过程中舒张功能障碍的其他测量指标相关。此外，4个月内的系列测量表明LA容积持续增加，适用于纵向心脏衰老研究。
