Zeng Biao, Liu Guang-Da, Zhang Bao-Bao, Wang Shan-shan, Ma Rui, Zhong Bei-Shan, He Bai-qiu, Liang Yan, Wu Fei-Hua
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
b Guangzhou Baiyun Shan Ming Xing Pharmaceutical Co. Ltd. , Guangzhou , China.
Nat Prod Res. 2016;30(9):1001-8. doi: 10.1080/14786419.2015.1095745. Epub 2015 Oct 29.
A new triterpene saponin, 3β,16β,23α,28β,30β-pentahydroxyl-olean-11,13(18)-dien-3β-yl-[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranoside, was named Clinoposaponin D (1), together with six known triterpene saponins, buddlejasaponin IVb (2), buddlejasaponin IVa (3), buddlejasaponin IV (4), clinopodisides D (5), 11α,16β,23,28-Tetrahydroxyolean-12-en-3β-yl-[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranoside (6) and prosaikogenin A (7), and two known triterpenes, saikogenin A (8) and saikogenin F (9) were isolated from Clinopodium chinense (Benth.) O. Kuntze. Their structures were elucidated on the basis of 1D, 2D NMR and MS analysis. Meanwhile, the effects of all compounds on rabbit platelet aggregation and thrombin time (TT) were investigated in vitro. Compounds 4 and 7 had significant promoting effects on platelet aggregation with EC50 value at 53.4 and 12.2 μM, respectively. In addition, the highest concentration (200 μM) of compounds 2 and 9 shortened TT by 20.6 and 25.1%, respectively.
从灯笼草（Clinopodium chinense (Benth.) O. Kuntze）中分离得到一种新的三萜皂苷，3β,16β,23α,28β,30β - 五羟基 - 齐墩果 - 11,13(18) - 二烯 - 3β - 基 - [β - D - 吡喃葡萄糖基 - (1→2)] - [β - D - 吡喃葡萄糖基 - (1→3)] - β - D - 吡喃岩藻糖苷，命名为风轮菜皂苷D（1），同时还得到了6种已知的三萜皂苷，醉鱼草皂苷IVb（2）、醉鱼草皂苷IVa（3）、醉鱼草皂苷IV（4）、风轮菜苷D（5）、11α,16β,23,28 - 四羟基齐墩果 - 12 - 烯 - 3β - 基 - [β - D - 吡喃葡萄糖基 - (1→2)] - [β - D - 吡喃葡萄糖基 - (1→3)] - β - D - 吡喃岩藻糖苷（6）和前柴胡皂苷A（7），以及2种已知的三萜，柴胡皂苷A（8）和柴胡皂苷F（9）。通过一维、二维核磁共振和质谱分析确定了它们的结构。同时，体外研究了所有化合物对兔血小板聚集和凝血酶时间（TT）的影响。化合物4和7对血小板聚集有显著促进作用，其半数有效浓度（EC50）分别为53.4和12.2 μM。此外，化合物2和9的最高浓度（200 μM）分别使TT缩短了20.6%和25.1%。
