School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035, People's Republic of China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100193, People's Republic of China.
Arch Pharm Res. 2018 Dec;41(12):1117-1130. doi: 10.1007/s12272-017-0943-9. Epub 2017 Sep 11.
Four new ursane-type triterpenoid saponins, clinopoursaponins A-D (1-4), six new oleanane-type triterpenoid saponins, clinopodiside VII-XII (5-10), as well as eight known triterpene analogues (11-18), were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. The structures of the new compounds were determined based on extensive spectral analyses, including 1D (H and C) and 2D NMR experiments (COSY, NOESY, HSQC, 2D TOCSY, HSQC-TOCSY and HMBC), HR-ESI-MS and chemical methods. Compounds 1-18 were evaluated for their protective effects against anoxia/reoxygenation-induced apoptosis in H9c2 cells and cytotoxicities against murine mammary carcinoma cell line 4T1. Compounds 8, 9 and 18 exhibited significant protective effects, while compound 1 exhibited cytotoxic activity with IC value of 7.4 μm compared to 7.6 μm for the positive control 10-hydroxycamptothecin.
从荆芥属植物（Benth.）O. Kuntze 的地上部分分离得到了四种新的五环三萜皂苷，即临床皂苷 A-D（1-4），六种新的齐墩果烷型三萜皂苷，即临床糖苷 VII-XII（5-10），以及八种已知的三萜类似物（11-18）。根据广泛的光谱分析，包括 1D（H 和 C）和 2D NMR 实验（COSY、NOESY、HSQC、2D TOCSY、HSQC-TOCSY 和 HMBC）、HR-ESI-MS 和化学方法，确定了新化合物的结构。对化合物 1-18 进行了抗缺氧/复氧诱导的 H9c2 细胞凋亡保护作用和对鼠乳腺癌细胞系 4T1 的细胞毒性评价。化合物 8、9 和 18 表现出显著的保护作用，而化合物 1 则表现出细胞毒性活性，IC 值为 7.4 μm，阳性对照 10-羟基喜树碱的 IC 值为 7.6 μm。
