a Department of Burn and Plastic Surgery, The First Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
b Department of Plastic Surgery, The First Hospital of Qin Huangdao , Qinhuangdao , Hebei , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3297-3305. doi: 10.1080/21691401.2019.1647224.
, perennial herb belongs to Araliaceae family used in traditional medicines to treat hypertension, thrombus, inflammation and cancer. In the present study, we biosynthesized goldnanoparticles using aqeous extract in a cost effective manner. The synthesized gold nanoparticle (SG-GNPs) were characterized using UV-Vis spec, HR-TEM, XRD, FTIR, SAED analysis. UV-Vis spectroscopic analysis showed a surface Plasmon resonance peak at 538 nm which does not reduce till 30 days of incubation. The results of HR-TEM, XRD and SAED confirm the spherical shape, crystalline nature and the size of the synthesized gold nanoparticles. The FTIR results prove that the biological components present in the had reduced the gold ions to synthesis gold nanoparticles. After characterization, the efficacy of SG-GNPS against the melanoma, a deadliest skin carcinoma, was assessed using B16 murine melanoma cells. The CC50 dose of SG-GNPs against B16 cells were assessed with MTT assay and the anticancer activity was evaluated using Rhodamine 123, H2DCFDA and dual staining techniques. The induction of apoptosis by SG-GNPs against melanoma cells were confirmed with q-PCR analysis. The results of staining techniques prove that SG-GNPs increase the reactive oxygen species and decreased the mitochondrial membrane potential. It is further confirmed by the results of q-PCR analysis which shows increased apoptotic Bid, Bad, Casp3, Casp 9 genes and decreased antiapoptotic Bcl2 gene expression in SG-GNPs treated cells. Our results authentically prove the biosynthesized SG-GNPs induces apoptosis in melanoma cells and it possesses anticancer property.
，多年生草本植物，属于五加科，用于传统医学治疗高血压、血栓、炎症和癌症。在本研究中，我们以经济有效的方式使用水提物生物合成了金纳米粒子。合成的金纳米颗粒（SG-GNPs）通过紫外可见光谱、高分辨率透射电子显微镜、X 射线衍射、傅里叶变换红外光谱、选区电子衍射分析进行了表征。紫外可见光谱分析显示表面等离子体共振峰在 538nm，在 30 天的孵育期内没有减少。高分辨率透射电子显微镜、X 射线衍射和选区电子衍射的结果证实了合成金纳米粒子的球形形状、结晶性质和尺寸。傅里叶变换红外光谱结果证明，存在于中的生物成分将金离子还原为合成金纳米粒子。在表征之后，使用 B16 小鼠黑色素瘤细胞评估了 SG-GNPS 对黑色素瘤的功效，黑色素瘤是最致命的皮肤癌。通过 MTT 测定法评估了 SG-GNPs 对 B16 细胞的 CC50 剂量，并用 Rhodamine 123、H2DCFDA 和双重染色技术评估了抗癌活性。通过 q-PCR 分析证实了 SG-GNPs 对黑色素瘤细胞的凋亡诱导作用。染色技术的结果证明，SG-GNPs 增加了活性氧物种并降低了线粒体膜电位。q-PCR 分析的结果进一步证实了这一点，该结果显示，在 SG-GNPs 处理的细胞中，促凋亡 Bid、Bad、Casp3、Casp9 基因表达增加，抗凋亡 Bcl2 基因表达降低。我们的结果确凿地证明了生物合成的 SG-GNPs 诱导黑色素瘤细胞凋亡，并具有抗癌特性。
