Etebari Kayvan, Osei-Amo Solomon, Blomberg Simon Phillip, Asgari Sassan
Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane QLD 4072 Australia.
Sci Rep. 2015 Oct 30;5:15968. doi: 10.1038/srep15968.
Recent discoveries regarding the importance of isomiRs have increased our understanding of the regulatory complexities of the miRNAome. Observed changes in the miRNA profiles in mosquitoes infected with flaviviruses have implicated small RNAs in the interactions between viruses and their vectors. Here we analysed the isomiR profiles of both uninfected and infected Aedes aegypti mosquitoes with the major human pathogen dengue virus (DENV). We found that several specific isomiRs were significantly altered in their abundance patterns in response to DENV infection potentially affecting their target repertoire. Notable among these were isomiR variants which displayed arm-switching. We also demonstrate that modifications to the 3p end of miRNAs are vastly more prevalent than those at the 5p ends. We also observed that in only 45% of Ae. aegypti miRNAs the most abundant read matches the exact sequence reported in miRBase. Further, we found positive correlations between the number of mature miRNA reads, pre-miRNA length, GC content and secondary structure minimum free energy with the number of isomiRs. The findings presented here provide some evidence that isomiR production is not a random phenomenon and may be important in DENV replication in its vector.
最近关于异时性微小RNA（isomiRs）重要性的发现增进了我们对微小RNA组调控复杂性的理解。在感染黄病毒的蚊子中观察到的微小RNA谱变化表明小RNA参与了病毒与其载体之间的相互作用。在此，我们分析了未感染和感染主要人类病原体登革病毒（DENV）的埃及伊蚊的异时性微小RNA谱。我们发现，几种特定的异时性微小RNA在丰度模式上因DENV感染而发生显著改变，这可能会影响它们的靶标库。其中值得注意的是显示臂转换的异时性微小RNA变体。我们还证明，微小RNA 3'端的修饰比5'端的修饰更为普遍。我们还观察到，在仅45%的埃及伊蚊微小RNA中，最丰富的读数与miRBase中报告的精确序列匹配。此外，我们发现成熟微小RNA读数的数量、前体微小RNA长度、GC含量和二级结构最小自由能与异时性微小RNA的数量之间存在正相关。此处呈现的研究结果提供了一些证据，表明异时性微小RNA的产生并非随机现象，可能在DENV在其载体中的复制中起重要作用。
