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使用高效表面增强拉曼散射（SERS）基底检测p53和表皮生长因子受体（EGFR）生物标志物

Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates.

作者信息

Owens Peter, Phillipson Nigel, Perumal Jayakumar, O'Connor Gerard M, Olivo Malini

机构信息

Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland.

School of Physics, National University Ireland, University Road, Galway, Ireland.

出版信息

Biosensors (Basel). 2015 Oct 28;5(4):664-77. doi: 10.3390/bios5040664.

DOI:10.3390/bios5040664
PMID:26516922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4697139/
Abstract

In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.

摘要

在本文中，我们描述了一种使用表面增强拉曼共振散射（SERRS）免疫测定法测定蛋白质浓度的方法。我们使用两种不同的拉曼活性连接体，4-氨基硫酚和6-巯基嘌呤，将其与高灵敏度表面增强拉曼散射（SERS）底物结合，并研究不同浓度的p53和表皮生长因子受体（EGFR）对拉曼光谱的影响。光谱中的扰动是由于蛋白质-抗体结合对拉曼连接体分子的影响，并且归因于局部机械应力的微小变化，这种变化通过SERRS得到增强。对于归因于C-S官能团的峰，这些影响最为显著，并且发现半高宽（FWHM）与蛋白质浓度成反比。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/4f44e933af58/biosensors-05-00664-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/25babaed1f27/biosensors-05-00664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/03078dde64e8/biosensors-05-00664-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/783e3de83f1d/biosensors-05-00664-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/07b18f96b24e/biosensors-05-00664-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/2d29fd456d0f/biosensors-05-00664-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/3eefe0237ec3/biosensors-05-00664-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/6d9a932630f2/biosensors-05-00664-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/a0744eb59245/biosensors-05-00664-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/78ef010a1ade/biosensors-05-00664-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/4f44e933af58/biosensors-05-00664-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/25babaed1f27/biosensors-05-00664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/03078dde64e8/biosensors-05-00664-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/783e3de83f1d/biosensors-05-00664-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/07b18f96b24e/biosensors-05-00664-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/2d29fd456d0f/biosensors-05-00664-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/3eefe0237ec3/biosensors-05-00664-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/6d9a932630f2/biosensors-05-00664-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/a0744eb59245/biosensors-05-00664-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/78ef010a1ade/biosensors-05-00664-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b22d/4697139/4f44e933af58/biosensors-05-00664-g010.jpg

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