Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA.
Mol Pharm. 2013 Jun 3;10(6):2291-8. doi: 10.1021/mp300634b. Epub 2013 May 9.
We present the application of a theranostic system combining Raman imaging and the photodynamic therapy (PDT) effect. The theranostic nanoplatform was created by loading the photosensitizer, protoporphyrin IX, onto a Raman-labeled gold nanostar. A cell-penetrating peptide, TAT, enhanced intracellular accumulation of the nanoparticles in order to improve their delivery and efficacy. The plasmonic gold nanostar platform was designed to increase the Raman signal via the surface-enhanced resonance Raman scattering (SERRS) effect. Theranostic SERS imaging and photodynamic therapy using this construct were demonstrated on BT-549 breast cancer cells. The TAT peptide allowed for effective Raman imaging and photosensitization with the nanoparticle construct after a 1 h incubation period. In the absence of the TAT peptide, nanoparticle accumulation in the cells was not sufficient to be observed by Raman imaging or to produce any photosensitization effect after this short incubation period. There was no cytotoxic effect observed after nanoparticle incubation, prior to light activation of the photosensitizer. This report shows the first application of combined SERS imaging and photosensitization from a theranostic nanoparticle construct.
我们展示了一种结合拉曼成像和光动力疗法（PDT）效应的治疗诊断系统的应用。该治疗诊断纳米平台是通过将光敏剂原卟啉 IX 加载到拉曼标记的金纳米星上来构建的。细胞穿透肽 TAT 增强了纳米颗粒在细胞内的积累，以提高其递送和疗效。等离子体金纳米星平台通过表面增强共振拉曼散射（SERRS）效应来增加拉曼信号。使用这种构建体在 BT-549 乳腺癌细胞上进行了治疗诊断 SERS 成像和光动力治疗。TAT 肽允许在 1 小时孵育期后，通过纳米颗粒构建体进行有效的拉曼成像和光敏化。在没有 TAT 肽的情况下，在如此短的孵育期后，纳米颗粒在细胞中的积累不足以通过拉曼成像观察到，也不会产生任何光敏化效应。在光激活光敏剂之前，纳米颗粒孵育后没有观察到细胞毒性效应。本报告首次展示了一种治疗诊断纳米颗粒构建体的联合 SERS 成像和光敏化的应用。
