Elvekrog Margaret M, Walter Peter
The Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, United States.
The Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, United States.
Curr Opin Chem Biol. 2015 Dec;29:79-86. doi: 10.1016/j.cbpa.2015.09.016. Epub 2015 Oct 30.
Most membrane and secretory proteins are delivered co-translationally to protein translocation channels in their destination membrane by the signal recognition particle (SRP) and its receptor. This co-translational molecular machinery is conserved across all kingdoms of life, though it varies in composition and function. Here we report recent progress towards understanding the mechanism of SRP function, focusing on findings about Escherichia coli SRP's conformational dynamics throughout the targeting process. These insights shed light on a key checkpoint in the targeting cycle: how SRP regulates engagement of an actively translating ribosome with the translocation machinery at the membrane.
大多数膜蛋白和分泌蛋白通过信号识别颗粒（SRP）及其受体在翻译过程中被递送至其目的地膜中的蛋白质转运通道。这种共翻译分子机制在所有生命王国中都是保守的，尽管其组成和功能有所不同。在这里，我们报告了在理解SRP功能机制方面的最新进展，重点关注关于大肠杆菌SRP在整个靶向过程中的构象动力学的研究结果。这些见解揭示了靶向循环中的一个关键检查点：SRP如何调节正在进行翻译的核糖体与膜上转运机制的结合。
