European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France.
Nat Struct Mol Biol. 2011 Jan;18(1):88-90. doi: 10.1038/nsmb.1952. Epub 2010 Dec 12.
We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.
我们通过 cryo-EM 报告了大肠杆菌信号识别颗粒 (SRP) 及其受体 FtsY 与正在翻译的核糖体结合的“早期”构象。FtsY 结合到 SRP RNA 的四螺旋环，而在这种构象中，SRP 蛋白的 NG 结构域和 FtsY 相互作用较弱。我们的结果表明，SRP RNA 四螺旋环和 Ffh NG 结构域的最佳定位导致了 FtsY 的募集。
