Zaia Mauricio Grecco, Oliveira Sandra Regina Pereira de, Castro Cynthia Aparecida de, Soares Edson Garcia, Afonso Ana, Monnazzi Luis Gustavo S, Peitl Filho Oscar, Faccioli Lúcia Helena, Anibal Fernanda de Freitas
Departamento de Morfologia e Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, SP, Brasil.
Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Mem Inst Oswaldo Cruz. 2015 Sep;110(6):726-31. doi: 10.1590/0074-02760150051.
The protective effect of infectious agents against allergic reactions has been thoroughly investigated. Current studies have demonstrated the ability of some helminths to modulate the immune response of infected hosts. The objective of the present study was to investigate the relationship between Toxocara canis infection and the development of an allergic response in mice immunised with ovalbumin (OVA). We determined the total and differential blood and bronchoalveolar lavage fluid cells using BALB/c mice as a model. To this end, the levels of interleukin (IL)-4, IL-5 and IL-10 and anti-OVA-IgE were measured using an ELISA. The inflammatory process in the lungs was observed using histology slides stained with haematoxylin and eosin. The results showed an increase in the total number of leukocytes and eosinophils in the blood of infected and immunised animals at 18 days after infection. We observed a slight lymphocytic inflammatory infiltrate in the portal space in all infected mice. Anti-OVA-IgE levels were detected in smaller proportions in the plasma of immunised and infected mice compared with mice that were only infected. Therefore, we concluded that T. canis potentiates inflammation in the lungs in response to OVA, although anti-OVA-IgE levels suggest a potential reduction of the inflammatory process through this mechanism.
感染因子对过敏反应的保护作用已得到充分研究。目前的研究表明，一些蠕虫具有调节受感染宿主免疫反应的能力。本研究的目的是调查犬弓首蛔虫感染与用卵清蛋白（OVA）免疫的小鼠过敏反应发展之间的关系。我们以BALB/c小鼠为模型，测定了血液及支气管肺泡灌洗液中的总细胞数和不同类型细胞数。为此，使用酶联免疫吸附测定法（ELISA）测量了白细胞介素（IL）-4、IL-5和IL-10以及抗OVA-IgE的水平。使用苏木精和伊红染色的组织学切片观察肺部的炎症过程。结果显示，感染并免疫的动物在感染后18天血液中的白细胞和嗜酸性粒细胞总数增加。我们在所有受感染小鼠的门管区观察到轻微的淋巴细胞炎性浸润。与仅受感染的小鼠相比，在免疫并受感染小鼠的血浆中检测到抗OVA-IgE的比例较小。因此，我们得出结论，犬弓首蛔虫会增强肺部对OVA的炎症反应，尽管抗OVA-IgE水平表明通过这种机制炎症过程可能会减轻。
