矿硃无义突变对酵母 URA1 mRNA 稳定性的影响。

Effect of ochre nonsense mutations on yeast URA1 mRNA stability.

机构信息

Laboratoire de Génétique Physiologique, Institut de Biologic Moléculaire et Cellulaire du C.N.R.S., 15 rue R. Descartes, 67084, Strasbourg Cedex, France.

出版信息

Curr Genet. 1984 May;8(4):277-82. doi: 10.1007/BF00419725.

DOI:10.1007/BF00419725

PMID:24177796

Abstract

The genetic map of 27 mutants of the URA1 yeast gene has been established by meiotic recombination and 16 nonsense mutations characterized. The half life of URA1 mRNA was determined by two independent methods in the wild-type and in two ochre mutants localized at each extremity of the genetic map. A halflife of 15 min was found for the wild-type and for one of the ochre mutants. This half-life was radically reduced in the other ochre mutant whereas the instantaneous rate of its mRNA synthesis remained constant. After subcloning various endonucleolytic fragments the coding sequence of the URA1 gene was restricted to a 1.65 kb fragment within a 5.7 kb yeast DNA segment. Direct visualization of the URA1 mRNA by Northern hybridization of denatured RNA with a URA1 specific DNA probe revealed a length of 1.5 kb.

摘要

已通过减数分裂重组建立了 27 个 URA1 酵母基因突变体的遗传图谱，并对 16 个无义突变进行了特征描述。野生型和两个定位在遗传图谱两端的赭石突变体中，通过两种独立的方法确定了 URA1 mRNA 的半衰期。野生型和一个赭石突变体的半衰期为 15 分钟。另一个赭石突变体的半衰期则大大缩短，而其 mRNA 合成的瞬时速率保持不变。在亚克隆各种内切核酸酶片段后，URA1 基因的编码序列被限制在一个包含在 5.7kb 酵母 DNA 片段内的 1.65kb 片段中。通过用 URA1 特异性 DNA 探针对变性 RNA 进行 Northern 杂交，直接观察到 URA1 mRNA 的长度为 1.5kb。