Gunsalus Kearney T W, Kumamoto Carol A
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA, 02111, USA.
Methods Mol Biol. 2016;1356:17-29. doi: 10.1007/978-1-4939-3052-4_2.
Gene expression profiling has become an important tool for determining gene functions, performing phenotypic analysis, and quantifying the differential expression of individual transcripts under various conditions. Candida albicans gene expression is highly responsive to environmental conditions and rapidly adapts to various niches within the host. Here, we describe a mouse model of gastrointestinal colonization with C. albicans, the measurement of colonization in fecal pellets, and the collection of samples for transcriptional profiling. We describe how to extract and purify RNA suitable for analysis via RT-qPCR or microarray.
基因表达谱分析已成为确定基因功能、进行表型分析以及量化各种条件下单个转录本差异表达的重要工具。白色念珠菌的基因表达对环境条件高度敏感，并能迅速适应宿主体内的各种生态位。在此，我们描述了一种白色念珠菌胃肠道定植的小鼠模型、粪便颗粒中定植情况的测量以及用于转录谱分析的样本采集。我们还描述了如何提取和纯化适用于通过逆转录定量聚合酶链反应（RT-qPCR）或微阵列进行分析的RNA。
