Kuykendall Randal J, Lockhart Shawn R
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Fungal Reference Laboratory, Mycotic Diseases Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop G-11, Atlanta, GA, 30333, USA.
Methods Mol Biol. 2016;1356:173-81. doi: 10.1007/978-1-4939-3052-4_13.
Antifungal susceptibility testing for Candida species is now widely accepted as a methodology to predict the success or failure of antifungal therapy for some antifungal/Candida species combinations. There are many different ways to perform susceptibility testing of antifungal agents, but broth microdilution has become the most popular over the last 10 years. This chapter describes in detail two methods for antifungal susceptibility testing of Candida species using the commercially available microbroth dilution tray (YeastOne(®)) and a commercially available gradient agar diffusion technique (Etest(®)) for isolates that appear resistant.
念珠菌属的抗真菌药敏试验作为一种预测某些抗真菌药物/念珠菌属组合抗真菌治疗成败的方法，现已被广泛接受。进行抗真菌药敏试验有许多不同的方法，但在过去10年中，肉汤微量稀释法已成为最常用的方法。本章详细描述了两种使用市售微量肉汤稀释板（YeastOne(®)）对念珠菌属进行抗真菌药敏试验的方法，以及一种针对出现耐药性的分离株使用市售梯度琼脂扩散技术（Etest(®)）的方法。
