Microbiotix Inc., Worcester, MA, USA.
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Methods Mol Biol. 2023;2658:17-34. doi: 10.1007/978-1-0716-3155-3_2.
Only three classes of contemporary antifungal drugs are routinely utilized in the clinic against filamentous fungal pathogens such as Aspergillus fumigatus. High-throughput phenotypic screens to identify small molecules with activity against filamentous fungi remain challenging due to the hyphal, biofilm-like growth morphology of these important organisms. In this chapter, we describe a protocol for utilizing a bioluminescent A. fumigatus strain for identifying small molecules that potentiate the activity of the triazole antifungal drug fluconazole. The assay holds great promise for identifying small molecules with activity against filamentous fungal pathogens.
临床上通常只有三类当代抗真菌药物可用于对抗烟曲霉等丝状真菌病原体。由于这些重要生物体呈菌丝状、类似生物膜的生长形态，因此进行针对丝状真菌的高通量表型筛选以鉴定具有活性的小分子仍然具有挑战性。在本章中，我们描述了一种利用发光烟曲霉菌株来鉴定可增强三唑类抗真菌药物氟康唑活性的小分子的方案。该测定法有望鉴定出针对丝状真菌病原体具有活性的小分子。
