Virella G
Clin Chim Acta. 1977 Feb 15;75(1):107-15. doi: 10.1016/0009-8981(77)90505-8.
Electrophoresis of lysozyme into agarose gel containing Micrococcus lysodeikticus causes lysis of the microorganism, allowing the development of two methods, one for quantitation ("lyso-rocket electrophoresis") and the other for electrophoretic characterization ("crossed lyso-electrophoresis") of lysozyme. The lyso-rocket technique provides an alternative to the method currently used for quantitative assay. By the use of crossed lyso-electrophoresis we have provided evidence, for the first time, of the electrophoretic heterogeneity of urinary lysozyme from patients with monocytic leukemia. We halve also documented the influence of concentration on the electrophoretic mobility of lysozyme.
将溶菌酶电泳到含有溶壁微球菌的琼脂糖凝胶中会导致该微生物裂解，由此产生了两种方法，一种用于溶菌酶定量（“溶菌火箭电泳”），另一种用于溶菌酶的电泳特性分析（“交叉溶菌电泳”）。溶菌火箭技术为目前用于定量测定的方法提供了一种替代方法。通过交叉溶菌电泳，我们首次提供了单核细胞白血病患者尿液溶菌酶电泳异质性的证据。我们还记录了浓度对溶菌酶电泳迁移率的影响。
